Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, J.; Wang, C.; Wu, Y.; Wu, M.; Wang, L.; Wang, Y.; Zang, J.
    Crystal structure of Sa239 reveals the structural basis for the activation of ribokinase by monovalent cations (2012), J. Struct. Biol., 177, 578-582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.15 expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.15 crystal structure of Sa239 in the absence of monovalent ions. The structure of Sa239 demonstrates that the C-terminal tail protrudes from the remaining part and interacts with the neighboring molecule, resulting in an dimerization form Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.15 2.19
-
 ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus
2.7.1.15 4.66
-
 ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.15 K+ monovalent ions such as K+ are able to enhance substrate affinity of Sa239 and activate the reaction catalyzed by Sa239 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.15 Staphylococcus aureus A0A0H2WZY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.15 ATP + D-ribose
-
 Staphylococcus aureus ADP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.15 dimer crystal structure Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.15 Sa239
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.15 30
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.15 20.65
-
 ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus
2.7.1.15 47.81
-
 ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.15 8
-
 assay at Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.15 4.42
-
 ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus
2.7.1.15 21.87
-
 ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus