Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagaki, M.; Ichijo, T.; Kobashi, R.; Yagihashi, Y.; Musashi, T.; Kawakami, J.; Ohya, N.; Gotoh, T.; Sagami, H.
    Substrate specificities of E- and Z-farnesyl diphosphate synthases with substrate analogs (2012), J. Mol. Catal. B, 80, 1-6.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expressed in Escherichia coli DH5alpha cells Geobacillus stearothermophilus
2.5.1.68 expressed in Escherichia coli BL21(DE3) cells Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.10 Y81S the mutant catalyzes the reaction between 8-tetrahydropyran-2-yloxygeranyl diphosphate and isopentenyl diphosphate, producing 12-tetrahydropyran-2-yloxyfarnesyl diphosphate with a yield of 12.3%. The mutant catalyzes the reaction between 8-acetoxygeranyl diphosphate with isopentenyl diphosphate, producing 12-acetoxyfarnesyl diphosphate and 16-acetoxygeranylgeranyl diphosphate with respective yields of 55.3% and 1.7% Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Geobacillus stearothermophilus Q08291
-
-
2.5.1.10 Geobacillus stearothermophilus ATCC 10149 Q08291
-
-
2.5.1.68 Thermobifida fusca Q47SS3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.68 His Trap column chromatography Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 8-acetoxygeranyl diphosphate + isopentenyl diphosphate
-
 Geobacillus stearothermophilus diphosphate + 12-acetoxyfarnesyl diphosphate 21.8% yield ?
2.5.1.10 8-acetoxygeranyl diphosphate + isopentenyl diphosphate
-
 Geobacillus stearothermophilus ATCC 10149 diphosphate + 12-acetoxyfarnesyl diphosphate 21.8% yield ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Geobacillus stearothermophilus diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Geobacillus stearothermophilus ATCC 10149 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.68 8-hydroxygeranyl diphosphate + isopentenyl diphosphate
-
 Thermobifida fusca (2Z)-12-hydroxyfarnesyl diphosphate + (2Z, 6Z)-16-hydroxygeranylgeranyl diphosphate + diphosphate 16.7% yield of (2Z)-12-hydroxyfarnesyl diphosphate, 6.6% yield of (2Z, 6Z)-16-hydroxygeranylgeranyl diphosphate ?
2.5.1.68 additional information the enzyme does not catalyze reactions between 8-tetrahydropyran-2-yloxygeranyl diphosphate and isopentenyl diphosphate Thermobifida fusca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 E-farnesyl diphosphate synthase
-
 Geobacillus stearothermophilus
2.5.1.10 E-FPP synthase
-
 Geobacillus stearothermophilus
2.5.1.68 Z-farnesyl diphosphate synthase
-
 Thermobifida fusca
2.5.1.68 Z-FPP synthase
-
 Thermobifida fusca