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Literature summary extracted from

  • Park, J.E.; Park, S.H.; Woo, J.Y.; Hwang, H.S.; Cha, J.; Lee, H.
    Enzymatic properties of a thermostable alpha-glucosidase from acidothermophilic crenarchaeon Sulfolobus tokodaii strain 7 (2013), J. Microbiol. Biotechnol., 23, 56-63.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.20 synthesis the enzyme can be potentially useful for starch hydrolysis as well for the novel synthesis of oligosaccharides in industry Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 expressed as a fusion protein in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 2.33
-
 maltopentaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 2.78
-
 maltotetraose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 3.11
-
 maltotriose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 3.18
-
 4-nitrophenyl alpha-D-glucopyranoside pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 4.4
-
 maltohexaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 5.09
-
 maltoheptaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 5.78
-
 panose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 10.9
-
 maltose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 17.3
-
 isomaltose pH 4.0, 80°C Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 70500
-
 x * 70500, SDS-PAGE Sulfurisphaera tokodaii
3.2.1.20 75600
-
 x * 75600, calculated from sequence Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Sulfurisphaera tokodaii F9VPH0
-
-
3.2.1.20 Sulfurisphaera tokodaii 7 F9VPH0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 12.7
-
 pH 4.0, 80°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.20 maltose + H2O
-
 Sulfurisphaera tokodaii 2 D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.20 maltotriose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 additional information exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. Sulfurisphaera tokodaii ?
-
 ?
3.2.1.20 additional information exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.20 panose + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 70500, SDS-PAGE Sulfurisphaera tokodaii
3.2.1.20 ? x * 75600, calculated from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 st2525
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 80
-
 assay at Sulfurisphaera tokodaii
3.2.1.20 95
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 75 110 75°C: about 50% of maximal activity, 110°C: about 75% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 95
-
 half-life: 144 min Sulfurisphaera tokodaii
3.2.1.20 100
-
 half-life: 40.1 min Sulfurisphaera tokodaii
3.2.1.20 105
-
 half-life: 7.75 min Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 0.0175
-
 4-nitrophenyl alpha-D-glucopyranoside pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.02
-
 maltopentaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.02
-
 maltohexaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.02
-
 maltoheptaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.02
-
 isomaltose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.02
-
 panose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.03
-
 maltotetraose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.04
-
 maltotriose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.11
-
 maltose pH 4.0, 80°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4
-
-
 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 3.5 5 pH 3.5: about 95% of maximal activity, pH 5.0: about 70% of maximal activity, potassium phosphate buffer Sulfurisphaera tokodaii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4 11 4°C, 30 min, the enzyme retains at least 85% of its maximal activity Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.20 0.0013
-
 isomaltose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0034
-
 panose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0038
-
 maltoheptaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0052
-
 maltohexaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0055
-
 4-nitrophenyl alpha-D-glucopyranoside pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0097
-
 maltose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0098
-
 maltopentaose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0113
-
 maltotetraose pH 4.0, 80°C Sulfurisphaera tokodaii
3.2.1.20 0.0136
-
 maltotriose pH 4.0, 80°C Sulfurisphaera tokodaii