EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.194 | Cu2+ | - |
Aspergillus sp. | |
3.2.1.194 | Fe2+ | - |
Aspergillus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.194 | Mg2+ | 50-100 mM, slightly improves activity | Aspergillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 56000 | - |
x * 56000, SDS-PAGE | Aspergillus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.194 | Aspergillus sp. | - |
- |
- |
3.2.1.194 | Aspergillus sp. 39g | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.194 | - |
Aspergillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 1.68 | - |
pH 6.0, 40°C | Aspergillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.194 | ginsenoside R1 + H2O | the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside | Aspergillus sp. | ginsenoside Rg1 + D-xylose | - |
? | |
3.2.1.194 | ginsenoside R1 + H2O | the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside | Aspergillus sp. 39g | ginsenoside Rg1 + D-xylose | - |
? | |
3.2.1.194 | ginsenoside Re + H2O | the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside | Aspergillus sp. | ginsenoside Rg1 + L-rhamnose | - |
? | |
3.2.1.194 | ginsenoside Re + H2O | the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside | Aspergillus sp. 39g | ginsenoside Rg1 + L-rhamnose | - |
? | |
3.2.1.194 | ginsenoside Rf + H2O | the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside | Aspergillus sp. | ginsenoside Rh1 + D-glucose | - |
? | |
3.2.1.194 | ginsenoside Rf + H2O | the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside | Aspergillus sp. 39g | ginsenoside Rh1 + D-glucose | - |
? | |
3.2.1.194 | ginsenoside Rg1 + H2O | the enzyme weakly hydrolyzes 6-O-beta-D-glucoside | Aspergillus sp. | ginsenoside F1 + D-glucose | - |
? | |
3.2.1.194 | ginsenoside Rg1 + H2O | the enzyme weakly hydrolyzes 6-O-beta-D-glucoside | Aspergillus sp. 39g | ginsenoside F1 + D-glucose | - |
? | |
3.2.1.194 | ginsenoside Rg2 + H2O | the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside | Aspergillus sp. | ginsenoside Rh1 + L-rhamnose | - |
? | |
3.2.1.194 | ginsenoside Rh1 + H2O | the enzyme weakly hydrolyzes 6-O-beta-D-glucoside | Aspergillus sp. | protopanaxatriol + D-glucose | - |
? | |
3.2.1.194 | additional information | ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd | Aspergillus sp. | ? | - |
? | |
3.2.1.194 | additional information | ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd | Aspergillus sp. 39g | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.194 | ? | x * 56000, SDS-PAGE | Aspergillus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 40 | - |
- |
Aspergillus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 20 | 60 | 20°C: about 70% of maximal activity, 60°C: about 80% of maximal activity | Aspergillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 6 | - |
- |
Aspergillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.194 | 3 | 8 | pH 3.0: about 70% of maximal activity, pH 8.0: about 80% of maximal activity | Aspergillus sp. |