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Literature summary extracted from

  • Connors, N.C.; Strohl, W.R.
    Partial purification and properties of carminomycin 4-0-methyltransferase from Streptomyces sp. strain C5 (1993), J. Gen. Microbiol., 139, 1353-1362.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.292 adenine 0.25 mM, 8% inhibition Streptomyces sp.
2.1.1.292 additional information homocysteine, adenosine, epsilon-rhodomycinone, daunomycin, and daunomycinone show little or no inhibitory activity Streptomyces sp.
2.1.1.292 S-adenosyl-L-homocysteine 0.025 mM, 87% inhibition Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.292 0.0005
-
 carminomycin pH 8.0, 37°C Streptomyces sp.
2.1.1.292 0.001
-
 13-dihydrocarminomycin pH 8.0, 37°C Streptomyces sp.
2.1.1.292 0.025
-
 S-adenosyl-L-methionine pH 8.0, 37°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.292 additional information no divalent cation requirement can be demonstrated for the partially purified enzyme Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.292 41000
-
 4 * 41000, SDS-PAGE Streptomyces sp.
2.1.1.292 166000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.292 S-adenosyl-L-methionine + 13-dihydrocarminomycin Streptomyces sp. terminal enzyme in the daunomycin biosynthesis pathway S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
2.1.1.292 S-adenosyl-L-methionine + carminomycin Streptomyces sp.
-
 S-adenosyl-L-homocysteine + daunorubicin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.292 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.292 partial Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.292 mycelium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.292 0.0194
-
 pH 8.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.292 additional information the purified enzyme does not catalyse methylation of the aglycones carminomycinone or 13-dihydrocarminomycinone Streptomyces sp. ?
-
 ?
2.1.1.292 S-adenosyl-L-methionine + 13-dihydrocarminomycin terminal enzyme in the daunomycin biosynthesis pathway Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
2.1.1.292 S-adenosyl-L-methionine + 13-dihydrocarminomycin the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
2.1.1.292 S-adenosyl-L-methionine + carminomycin
-
 Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicin
-
 ?
2.1.1.292 S-adenosyl-L-methionine + carminomycin the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.292 tetramer 4 * 41000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
2.1.1.292 DnrK
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.292 37
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.292 30 45 30°C: about 90% of maximal activity, 45°C: about 90% of maximal activity Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.292 8
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.292 7.5 8 pH 7.5: 40% of maximal activity, pH 8.0: optimum. No pH values above pH 8.0 are evaluated due to the inherent instability of S-adenosyl-L-methionine at alkaline pH Streptomyces sp.