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Literature summary extracted from

  • Connors, N.C.; Bartel, P.L.; Strohl, W.R.
    Biosynthesis of anthracyclines: carminomycin 4-0-methyltransferase, the terminal enzymic step in the formation of daunomycin (1990), J. Gen. Microbiol., 136, 1895-1898.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.292 S-adenosyl-L-methionine + carminomycin Streptomyces sp. with the corresponding aglycones, carminomycinone and 13-dihydrocarminomycinone, as substrates, no methylated products are detected. Other 4-hydroxyanthracyclines such as aklavin and aclacinomycin A, and 4-hydroxyanthracyclinones such as erhodomycinone and aklavinone, are not substrates for the 4-O-methyltransferase. These reaction specificities indicate that glycosylation of the anthracyclinone molecule must occur before 4-O-methylation, which means that 4-O-methylation of carminomycin is probably the terminal step in the biosynthesis of daunomycin, and that daunomycinone is not an intermediate in the pathway S-adenosyl-L-homocysteine + daunomycin
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Organism

EC Number Organism UniProt Comment Textmining
2.1.1.292 Streptomyces sp. Q55216
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.292 S-adenosyl-L-methionine + 13-dihydrocarminomycin
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Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicinol
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2.1.1.292 S-adenosyl-L-methionine + carminomycin
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Streptomyces sp. S-adenosyl-L-homocysteine + daunomycin
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?
2.1.1.292 S-adenosyl-L-methionine + carminomycin with the corresponding aglycones, carminomycinone and 13-dihydrocarminomycinone, as substrates, no methylated products are detected. Other 4-hydroxyanthracyclines such as aklavin and aclacinomycin A, and 4-hydroxyanthracyclinones such as erhodomycinone and aklavinone, are not substrates for the 4-O-methyltransferase. These reaction specificities indicate that glycosylation of the anthracyclinone molecule must occur before 4-O-methylation, which means that 4-O-methylation of carminomycin is probably the terminal step in the biosynthesis of daunomycin, and that daunomycinone is not an intermediate in the pathway Streptomyces sp. S-adenosyl-L-homocysteine + daunomycin
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?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.292 COMT
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Streptomyces sp.

General Information

EC Number General Information Comment Organism
2.1.1.292 physiological function the enzyme catalyzes the terminal enzymic step in the formation of daunomycin Streptomyces sp.