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Literature summary extracted from

  • Katoh, H.; Tamaki, H.; Tokutake, Y.; Hanada, S.; Chohnan, S.
    Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei (2013), J. Biosci. Bioeng., 115, 372-376.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.36 acetyl-CoA activates enzyme activity 1.64fold Methanospirillum hungatei
6.3.2.36 CoA activates enzyme activity 3.12fold Methanospirillum hungatei
6.3.2.36 malonyl-CoA activates enzyme activity 1.36fold Methanospirillum hungatei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.169 expression in Escherichia coli Methanospirillum hungatei
6.3.2.36 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109 Methanospirillum hungatei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.169 malonyl-CoA 0.2 mM, 83% residual activity Methanospirillum hungatei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.169 Ca2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
2.7.1.169 Co2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
2.7.1.169 Fe2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
2.7.1.169 Mg2+ maximum activity in presence of Mg2+ Methanospirillum hungatei
2.7.1.169 Mn2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
2.7.1.169 additional information not accepted: Cu2+ Methanospirillum hungatei
2.7.1.169 Ni2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
2.7.1.169 Zn2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
6.3.2.36 Mg2+ required Methanospirillum hungatei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.36 ATP + (R)-4-phosphopantoate + beta-alanine Methanospirillum hungatei
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
?
6.3.2.36 ATP + (R)-4-phosphopantoate + beta-alanine Methanospirillum hungatei JF-1
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.169 Methanospirillum hungatei Q2FUB2
-
-
6.3.2.36 Methanospirillum hungatei Q2FUA9
-
-
6.3.2.36 Methanospirillum hungatei JF-1 Q2FUA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.36 recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration Methanospirillum hungatei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.169 ATP + (R)-pantoate
-
Methanospirillum hungatei ADP + (R)-phosphopantoate
-
?
2.7.1.169 UTP + (R)-pantoate 52.7% of the activity with ATP Methanospirillum hungatei UDP + (R)-phosphopantoate
-
?
6.3.2.36 ATP + (R)-4-phosphopantoate + beta-alanine
-
Methanospirillum hungatei AMP + diphosphate + (R)-4'-phosphopantothenate
-
?
6.3.2.36 ATP + (R)-4-phosphopantoate + beta-alanine
-
Methanospirillum hungatei JF-1 AMP + diphosphate + (R)-4'-phosphopantothenate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.169 COG1829
-
Methanospirillum hungatei
2.7.1.169 Mhun_0831
-
Methanospirillum hungatei
6.3.2.36 Mhun_0832
-
Methanospirillum hungatei
6.3.2.36 phosphopantothenate synthetase
-
Methanospirillum hungatei
6.3.2.36 Pps
-
Methanospirillum hungatei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.169 40
-
-
Methanospirillum hungatei
6.3.2.36 30
-
assay at Methanospirillum hungatei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.169 60
-
no residual activity Methanospirillum hungatei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.169 8.5
-
-
Methanospirillum hungatei
6.3.2.36 7.5
-
assay at Methanospirillum hungatei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.169 8 9.5 more than 80% of maximum activity Methanospirillum hungatei

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.36 ATP
-
Methanospirillum hungatei

General Information

EC Number General Information Comment Organism
2.7.1.169 physiological function coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively Methanospirillum hungatei