EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.36 | acetyl-CoA | activates enzyme activity 1.64fold | Methanospirillum hungatei | |
6.3.2.36 | CoA | activates enzyme activity 3.12fold | Methanospirillum hungatei | |
6.3.2.36 | malonyl-CoA | activates enzyme activity 1.36fold | Methanospirillum hungatei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.169 | expression in Escherichia coli | Methanospirillum hungatei |
6.3.2.36 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109 | Methanospirillum hungatei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.169 | malonyl-CoA | 0.2 mM, 83% residual activity | Methanospirillum hungatei |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.169 | Ca2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | Co2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | Fe2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | Mg2+ | maximum activity in presence of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | Mn2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | additional information | not accepted: Cu2+ | Methanospirillum hungatei | |
2.7.1.169 | Ni2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
2.7.1.169 | Zn2+ | broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ | Methanospirillum hungatei | |
6.3.2.36 | Mg2+ | required | Methanospirillum hungatei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.36 | ATP + (R)-4-phosphopantoate + beta-alanine | Methanospirillum hungatei | - |
AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
6.3.2.36 | ATP + (R)-4-phosphopantoate + beta-alanine | Methanospirillum hungatei JF-1 | - |
AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.169 | Methanospirillum hungatei | Q2FUB2 | - |
- |
6.3.2.36 | Methanospirillum hungatei | Q2FUA9 | - |
- |
6.3.2.36 | Methanospirillum hungatei JF-1 | Q2FUA9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.36 | recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration | Methanospirillum hungatei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.169 | ATP + (R)-pantoate | - |
Methanospirillum hungatei | ADP + (R)-phosphopantoate | - |
? | |
2.7.1.169 | UTP + (R)-pantoate | 52.7% of the activity with ATP | Methanospirillum hungatei | UDP + (R)-phosphopantoate | - |
? | |
6.3.2.36 | ATP + (R)-4-phosphopantoate + beta-alanine | - |
Methanospirillum hungatei | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
6.3.2.36 | ATP + (R)-4-phosphopantoate + beta-alanine | - |
Methanospirillum hungatei JF-1 | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.169 | COG1829 | - |
Methanospirillum hungatei |
2.7.1.169 | Mhun_0831 | - |
Methanospirillum hungatei |
6.3.2.36 | Mhun_0832 | - |
Methanospirillum hungatei |
6.3.2.36 | phosphopantothenate synthetase | - |
Methanospirillum hungatei |
6.3.2.36 | Pps | - |
Methanospirillum hungatei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.169 | 40 | - |
- |
Methanospirillum hungatei |
6.3.2.36 | 30 | - |
assay at | Methanospirillum hungatei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.169 | 60 | - |
no residual activity | Methanospirillum hungatei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.169 | 8.5 | - |
- |
Methanospirillum hungatei |
6.3.2.36 | 7.5 | - |
assay at | Methanospirillum hungatei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.169 | 8 | 9.5 | more than 80% of maximum activity | Methanospirillum hungatei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.36 | ATP | - |
Methanospirillum hungatei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.169 | physiological function | coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively | Methanospirillum hungatei |