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Literature summary extracted from

  • Sasaki, K.; Tsurumaru, Y.; Yamamoto, H.; Yazaki, K.
    Molecular characterization of a membrane-bound prenyltransferase specific for isoflavone from Sophora flavescens (2011), J. Biol. Chem., 286, 24125-24134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.36 gene GmG4DT, sequence comparison and phylogenetic tree Glycine max
2.5.1.70
-
 Sophora flavescens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.70 additional information construction of chimeric enzymes between narigenin 8-dimethylallyltransferase and isoflavone-specific prenyltransferase G6DT responsible for the prenylation of the genistein at the 6-position, by recombination of amino acid sequences within the loop domains. Chimera SfG2N possesses purely narigenin 8-dimethylallyltransferase activity despite the absence of the N-terminal sequence derived from narigenin 8-dimethylallyltransferase, 243 amino acids. Chimera SfG3N possesses only isoflavone-specific prenyltransferase G6DT activity, although its 33% of the C-terminus is from narigenin 8-dimethylallyltransferase polypeptide. Neither chimeric enzyme can catalyze the prenylation reaction for other types of flavonoid such as kaempferol, apigenin, naringenin chalcone Sophora flavescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.70 0.025
-
 3,3-dimethylallyl diphosphate pH 9.0, 30°C Sophora flavescens
2.5.1.70 0.05
-
 (2S)-naringenin pH 9.0, 30°C Sophora flavescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.36 membrane bound Glycine max 16020
-
2.5.1.70 membrane
-
 Sophora flavescens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.36 Glycine max B9A1Q4
-
-
2.5.1.70 Sophora flavescens F8WPJ0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.36 dimethylallyl diphosphate + genistein
-
 Glycine max diphosphate + ?
-
 ?
2.5.1.36 additional information glycinol 4-dimethylallyltransferase G4DT is specific for pterocarpan substrates Glycine max ?
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-liquiritigenin
-
 Sophora flavescens diphosphate + isobavachin
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin
-
 Sophora flavescens diphosphate + sophoraflavanone B
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.36 G4DT
-
 Glycine max
2.5.1.36 glycinol 4-dimethylallyltransferase
-
 Glycine max
2.5.1.70 N8DT-3
-
 Sophora flavescens

General Information

EC Number General Information Comment Organism
2.5.1.36 metabolism the prenyltransferase is the key enzyme in the biosynthesis of prenylated flavonoids by catalyzing a rate-limiting step, i.e. the coupling process of two major metabolic pathways, the isoprenoid pathway and shikimate/polyketide pathway Glycine max