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Literature summary extracted from

  • Lebska, M.; Ciesielski, A.; Szymona, L.; Godecka, L.; Lewandowska-Gnatowska, E.; Szczegielniak, J.; Muszynska, G.
    Phosphorylation of maize eukaryotic translation initiation factor 5A (eIF5A) by casein kinase 2: Identification of phosphorylated residue and influence on intracellular localization of eIF5A (2010), J. Biol. Chem., 285, 6217-6226.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 cloning of catalytic subunit alpha of CK2, i.e. ZmCK2alpha-1, and expression as GST-tagged enzyme in Escherichia coli strain BL21(DE3) Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 nucleus
-
 Zea mays 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ required Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A Zea mays
-
 ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A Zea mays Mona
-
 ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Zea mays
-
-
-
2.7.11.1 Zea mays Mona
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 native CK2 from seedlings by ammonium sulfate fractionation, cation exchange chromatography, ultrafiltration, dialysis, anion exchange chromatography, again ultrafiltration, and gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 leaf
-
 Zea mays
-
2.7.11.1 seedling
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A
-
 Zea mays ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated Zea mays ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A
-
 Zea mays Mona ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
2.7.11.1 ATP + maize eukaryotic translation initiation factor 5A phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated Zea mays Mona ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 casein kinase 2
-
 Zea mays
2.7.11.1 CK2
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 8
-
 assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Zea mays

General Information

EC Number General Information Comment Organism
2.7.11.1 physiological function phosphorylation of Ser2 plays a role in regulation of nucleocytoplasmic shuttling of eukaryotic translation initiation factor 5A in plant cells Zea mays