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Literature summary extracted from

  • Kennedy, E.M.; Gavegnano, C.; Nguyen, L.; Slater, R.; Lucas, A.; Fromentin, E.; Schinazi, R.F.; Kim, B.
    Ribonucleoside triphosphates as substrate of human immunodeficiency virus type 1 reverse transcriptase in human macrophages (2010), J. Biol. Chem., 285, 39380-39391.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.49 expression of N-terminal His6-tagged p66/p66 homodimer HIV-1 RT in Escherichia coli strain BL21 (DE3) Human immunodeficiency virus 1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.49 3'-deoxyadenosine inhibits HIV-1 proviral DNA synthesis in human macrophages more efficiently than in CD4+ T cells Human immunodeficiency virus 1
2.7.7.49 additional information ribonucleoside chain terminators may be a class of anti-HIV-1 agents specifically targeting viral macrophage infection Human immunodeficiency virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.49 additional information
-
 additional information steady-state kinetic analysis of HIV-1 RT rNTP incorporation Human immunodeficiency virus 1
2.7.7.49 0.0087
-
 dATP pH and temperature not specified in the publication Human immunodeficiency virus 1
2.7.7.49 0.059
-
 dGTP pH and temperature not specified in the publication Human immunodeficiency virus 1
2.7.7.49 0.155
-
 dCTP pH and temperature not specified in the publication Human immunodeficiency virus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.49 dATP + DNAn Human immunodeficiency virus 1
-
 diphosphate + DNAn+1
-
 ?
2.7.7.49 dCTP + DNAn Human immunodeficiency virus 1
-
 diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn Human immunodeficiency virus 1
-
 diphosphate + DNAn+1
-
 ?
2.7.7.49 dGTP + DNAn Human immunodeficiency virus 1
-
 diphosphate + DNAn+1
-
 ?
2.7.7.49 additional information Human immunodeficiency virus 1 ribonucleoside triphosphate are efficiently incorporated into DNA in the macrophage but not in the T cell environment, detailed overview. HIV-1 RT initiates both (-)- and (+)-proviral DNA synthesis using RNA primers (e.g. tRNA3Lys and polypurine tract RNA primer) containing 3'-end ribonucleoside monophosphates during viral replication ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 Human immunodeficiency virus 1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.49 recombinant N-terminal His6-tagged p66/p66 homodimer HIV-1 RT from Escherichia coli strain BL21 (DE3) by nickel affinity, anion exchange, and cation exchange chromatography Human immunodeficiency virus 1

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 dATP + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 dCTP + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn Ext-T DNA 23-mer primer annealed to the RNA 40-mer template Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 dGTP + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 additional information ribonucleoside triphosphate are efficiently incorporated into DNA in the macrophage but not in the T cell environment, detailed overview. HIV-1 RT initiates both (-)- and (+)-proviral DNA synthesis using RNA primers (e.g. tRNA3Lys and polypurine tract RNA primer) containing 3'-end ribonucleoside monophosphates during viral replication Human immunodeficiency virus 1 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.49 0.000083
-
 dCTP pH and temperature not specified in the publication Human immunodeficiency virus 1
2.7.7.49 0.00075
-
 dATP pH and temperature not specified in the publication Human immunodeficiency virus 1
2.7.7.49 0.00088
-
 dGTP pH and temperature not specified in the publication Human immunodeficiency virus 1