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Literature summary extracted from

  • Schmitt, M.W.; Venkatesan, R.N.; Pillaire, M.J.; Hoffmann, J.S.; Sidorova, J.M.; Loeb, L.A.
    Active site mutations in mammalian DNA polymerase delta alter accuracy and replication fork progression (2010), J. Biol. Chem., 285, 32264-32272.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 proliferating cell nuclear antigen PCNA, the interaction between pol delta and PCNA is mediated predominantly by the p50 accessory subunit of pol delta and not the p125 catalytic subunit Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 L606G site-directed mutagenesis, the mutant shows increased polymerase activity and slightly reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606G is highly error prone, incorporating single noncomplementary nucleotides at a high frequency during DNA synthesis Homo sapiens
2.7.7.7 L606K site-directed mutagenesis, the mutant shows increased polymerase activity and reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606K is extremely accurate, with a higher fidelity of single nucleotide incorporation by the active site than that of wild-type pol delta, it does not catalyze detectable nucleotide mis-insertion even with nucleotide concentrations as high as 4 mM, but pol delta L606K mutant is impaired in the bypass of DNA adducts Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 EDTA
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information Michaelis-Menten kinetics and single nucleotide kinetics of wild-type and mutat enzymes, overview Homo sapiens
2.7.7.7 0.00249
-
 dCTP mutant L606K, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
2.7.7.7 0.00464
-
 dCTP wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
2.7.7.7 0.00792
-
 dCTP mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
2.7.7.7 0.175
-
 dTTP wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
2.7.7.7 0.211
-
 dTTP mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Homo sapiens
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 dCTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 dTTP + DNAn activity assay with plus-strand m13 DNA annealed to 5'-32P end-labeled primer 5'-GCTGTTGGGAAGGGCGATCG-3' Homo sapiens diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 tetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase delta
-
 Homo sapiens
2.7.7.7 pol delta
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5 7.9 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.7.7 malfunction enhanced fidelity of base selection by a polymerase active site can result in impaired lesion bypass and delayed replication fork progression Homo sapiens
2.7.7.7 physiological function 3'-> 5'-exonucleolytic proofreading activity Homo sapiens