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Literature summary extracted from

  • Sun, X.E.; Sharling, L.; Muthalagi, M.; Mudeppa, D.G.; Pankiewicz, K.W.; Felczak, K.; Rathod, P.K.; Mead, J.; Striepen, B.; Hedstrom, L.
    Prodrug activation by Cryptosporidium thymidine kinase (2010), J. Biol. Chem., 285, 15916-15922.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.21 dCDP dCDP activates the enzyme 2.5old Cryptosporidium parvum
2.7.1.21 dCTP dCTP is not a phosphate donor, but instead activates the enzyme 7fold Cryptosporidium parvum
2.7.1.21 additional information the enzyme is not activated by dATP or dGTP Cryptosporidium parvum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Cryptosporidium parvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dTTP
-
Cryptosporidium parvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.022
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.032
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.04
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.048
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.05
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.118
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.14
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.29
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 23300
-
3 * 23300, calculated from amino acid sequence Cryptosporidium parvum
2.7.1.21 70000
-
gel filtration Cryptosporidium parvum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Cryptosporidium parvum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 3'-aminothymidine-Sepharose resin column chromatography and HisTrap column chromatography Cryptosporidium parvum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2',3'-didehydro-2',3'-dideoxythymidine 12% activity compared to thymidine Cryptosporidium parvum ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-phosphate
-
?
2.7.1.21 ATP + 3'-azido-2',3'-dideoxythymidine 60% activity compared to thymidine Cryptosporidium parvum ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
?
2.7.1.21 ATP + 5-bromodeoxyuridine 84% activity compared to thymidine Cryptosporidium parvum ADP + 5-bromodeoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + 5-chlorodeoxyuridine 110% activity compared to thymidine Cryptosporidium parvum ADP + 5-chlorodeoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + 5-fluoro-2'-deoxyuridine good substrate, 120% activity compared to thymidine Cryptosporidium parvum ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + 5-iododeoxyuridine 89% activity compared to thymidine Cryptosporidium parvum ADP + 5-iododeoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + beta-L-thymidine less than 5% activity compared to thymidine Cryptosporidium parvum ADP + beta-L-thymidine 5'-phosphate
-
?
2.7.1.21 ATP + deoxycytidine 7% activity compared to thymidine Cryptosporidium parvum ADP + dCMP
-
?
2.7.1.21 ATP + deoxyuridine 69% activity compared to thymidine Cryptosporidium parvum ADP + dUMP
-
?
2.7.1.21 ATP + guanosine 1% activity compared to thymidine Cryptosporidium parvum ADP + GMP
-
?
2.7.1.21 ATP + thymidine 100% activity Cryptosporidium parvum ADP + dTMP
-
?
2.7.1.21 ATP + trifluoromethylthymidine good substrate, 130% activity compared to thymidine Cryptosporidium parvum ADP + trifluoromethylthymidine 5’-phosphate
-
?
2.7.1.21 dGTP + thymidine 99% activity compared to ATP Cryptosporidium parvum dGDP + dTMP
-
?
2.7.1.21 GTP + thymidine 42% activity compared to ATP Cryptosporidium parvum GDP + dTMP
-
?
2.7.1.21 additional information CTP, diphosphate, uridine, adenosine, cytosine, deoxyguanosine, deoxyguanosine, dTMP, thymine 1-beta-D-arabinofuranoside, 1-beta-D-arabinofuranosylcytosine, acyclovir, and 5-iodo-2'-deoxycytidine are no substrates Cryptosporidium parvum ?
-
?
2.7.1.21 UTP + thymidine 17% activity compared to ATP Cryptosporidium parvum UDP + dTMP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 trimer 3 * 23300, calculated from amino acid sequence Cryptosporidium parvum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.21 0.007
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.03
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.17
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.24
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.28
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.28
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.36
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.53
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.21 0.0024
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 0.064
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 2.3
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 4.5
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 4.8
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 5
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 5.8
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
2.7.1.21 6.4
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum