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Literature summary extracted from

  • Ownby, K., Xu, H. and White, R.H.
    A Methanocaldococcus jannaschii archaeal signature gene encodes for a 5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase. A new enzyme in purine biosynthesis (2005), J. Biol. Chem., 280, 10881-10887.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.23 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.23 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 Methanocaldococcus jannaschii
6.3.4.23 ADP
-
 Methanocaldococcus jannaschii
6.3.4.23 additional information phosphonoacetaldehyde (20 mM) is not an inhibitor or substrate for the reaction Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.23 Mg2+ Mg2+ is the best divalent metal in supporting the reaction, with Mn2+ being 87% as active as Mg2+ Methanocaldococcus jannaschii
6.3.4.23 Mn2+ Mg2+ is the best divalent metal in supporting the reaction, with Mn2+ being 87% as active as Mg2+ Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.23 ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide Methanocaldococcus jannaschii catalyses a step in the synthesis of purine nucleotides ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.23 Methanocaldococcus jannaschii Q57600
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.23
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.23 1.9
-
 pH 7.3, 70°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.23 ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide catalyses a step in the synthesis of purine nucleotides Methanocaldococcus jannaschii ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
6.3.4.23 ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide no other nucleotide, when substituted for ATP, can function as a substrate Methanocaldococcus jannaschii ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
6.3.4.23 formylphosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 Methanocaldococcus jannaschii 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ?
-
 ?
6.3.4.23 additional information phosphonoacetaldehyde (20 mM) is not an inhibitor or substrate for the reaction Methanocaldococcus jannaschii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.23 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase
-
 Methanocaldococcus jannaschii
6.3.4.23 5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase
-
 Methanocaldococcus jannaschii
6.3.4.23 PurP
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.23 70
-
 assay at Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.23 100
-
 the enzyme maintains 100% of activity when heated for 15 min at temperatures up to 100°C, but it loses all activity when heated above 100°C for 15 min Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.23 6.3
-
-
 Methanocaldococcus jannaschii
6.3.4.23 7.3
-
 assay at Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.23 5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity Methanocaldococcus jannaschii