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Literature summary extracted from

  • Ohnuma, S.; Suzuki, M.; Nishino, T.
    Archaebacterial ether-linked lipid biosynthetic gene. Expression cloning, sequencing, and characterization of geranylgeranyl diphosphate synthase (1994), J. Biol. Chem., 269, 14792-14797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29
-
 Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 36873
-
 x * 36873, calculated from sequence Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Sulfolobus acidocaldarius biosynthesis of geranylgeranyl diphosphate, a precursor for the ether-linked lipids diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Sulfolobus acidocaldarius DSM 639 biosynthesis of geranylgeranyl diphosphate, a precursor for the ether-linked lipids diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Sulfolobus acidocaldarius P39464
-
-
2.5.1.29 Sulfolobus acidocaldarius DSM 639 P39464
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 partial Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.29 0.0087
-
 pH and temperature not specified in the publication Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate biosynthesis of geranylgeranyl diphosphate, a precursor for the ether-linked lipids Sulfolobus acidocaldarius diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate (2Z,6E)-farnesyl diphosphate is not a substrate. Dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are good substrates, with maximal activity for dimethylallyl diphosphate Sulfolobus acidocaldarius diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are, in decreasing order of activity, acceptable as allylic substrates to produce geranylgeranyl diphosphate. When dimethylallyl diphosphate or geranyl diphosphate are the allylic substrates, a significant amount of mixture of the products is shorter than geranylgeranyl diphosphate. (2Z,6E)-farnesyl diphosphate is not a substrate Sulfolobus acidocaldarius diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate biosynthesis of geranylgeranyl diphosphate, a precursor for the ether-linked lipids Sulfolobus acidocaldarius DSM 639 diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are good substrates, with maximal activity for dimethylallyl diphosphate Sulfolobus acidocaldarius geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are, in decreasing order of activity, acceptable as allylic substrates to produce geranylgeranyl diphosphate. When dimethylallyl diphosphate or geranyl diphosphate are the allylic substrates, a significant amount of mixture of the products is shorter than geranylgeranyl diphosphate. (2Z,6E)-farnesyl diphosphate is not a substrate Sulfolobus acidocaldarius geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are good substrates, with maximal activity for dimethylallyl diphosphate Sulfolobus acidocaldarius DSM 639 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are, in decreasing order of activity, acceptable as allylic substrates to produce geranylgeranyl diphosphate. When dimethylallyl diphosphate or geranyl diphosphate are the allylic substrates, a significant amount of mixture of the products is shorter than geranylgeranyl diphosphate. (2Z,6E)-farnesyl diphosphate is not a substrate Sulfolobus acidocaldarius DSM 639 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are good substrates, with maximal activity for dimethylallyl diphosphate Sulfolobus acidocaldarius geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are, in decreasing order of activity, acceptable as allylic substrates to produce geranylgeranyl diphosphate. When dimethylallyl diphosphate or geranyl diphosphate are the allylic substrates, a significant amount of mixture of the products is shorter than geranylgeranyl diphosphate. (2Z,6E)-farnesyl diphosphate is not a substrate Sulfolobus acidocaldarius geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are good substrates, with maximal activity for dimethylallyl diphosphate Sulfolobus acidocaldarius DSM 639 geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate dimethylallyl diphosphate, geranyl diphosphate, and (2E,6E)-farnesyl diphosphate are, in decreasing order of activity, acceptable as allylic substrates to produce geranylgeranyl diphosphate. When dimethylallyl diphosphate or geranyl diphosphate are the allylic substrates, a significant amount of mixture of the products is shorter than geranylgeranyl diphosphate. (2Z,6E)-farnesyl diphosphate is not a substrate Sulfolobus acidocaldarius DSM 639 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 ? x * 36873, calculated from sequence Sulfolobus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
2.5.1.29 GGPP synthase
-
 Sulfolobus acidocaldarius
2.5.1.29 Saci_0092 locus name Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.29 60 70 100 min, most of the activity remains Sulfolobus acidocaldarius
2.5.1.29 60 70 100 min, most of the activity for geranylgeranyl synthase remains even after heating at 60 to 70°C for 100 min Sulfolobus acidocaldarius
2.5.1.29 90
-
 10 min, 90% loss of activity Sulfolobus acidocaldarius
2.5.1.29 90
-
 10 min, 90% of the enzyme activity is lost Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 5.8
-
-
 Sulfolobus acidocaldarius
2.5.1.29 5.9
-
-
 Sulfolobus acidocaldarius

General Information

EC Number General Information Comment Organism
2.5.1.29 physiological function biosynthesis of geranylgeranyl diphosphate, a precursor for the ether-linked lipids Sulfolobus acidocaldarius