Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hirano, Y.; Suzuki, T.; Matsumoto, T.; Ishihara, Y.; Takaki, Y.; Kono, M.; Dohmae, N.; Tsuji, S.
    Disulphide linkage in mouse ST6Gal-I: determination of linkage positions and mutant analysis (2012), J. Biochem., 151, 197-203.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expressed in COS-7 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.1 C139A the mutant has almost the same activity (98%) and specificity as those of native enzyme Mus musculus
2.4.3.1 C139S the mutant has almost the same activity (94%) and specificity as those of native enzyme Mus musculus
2.4.3.1 C181S no activity Mus musculus
2.4.3.1 C350A no activity Mus musculus
2.4.3.1 C350S no activity Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 soluble
-
 Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 70000
-
 1 * 70000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Mus musculus Q64685
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.1 glycoprotein the enzyme has two N-glycosylation sites Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 IgG Sepharose column chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine 100% activity Mus musculus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + Galbeta(1->3)GalNAc 10% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Mus musculus CMP + NeuAcalpha(2->6)Galbeta(1->3)GalNAc
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + Galbeta(1->3)GlcNAc 2% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Mus musculus CMP + NeuAcalpha(2->6)Galbeta(1->3)GlcNAc
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lacto-N-neotetraose 108% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Mus musculus CMP + ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lacto-N-tetraose 35% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Mus musculus CMP + ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose 14% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Mus musculus CMP + 6'-sialyllactose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 monomer 1 * 70000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha2,6-sialyltransferase
-
 Mus musculus
2.4.3.1 ST6Gal-I
-
 Mus musculus