EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.1 | expressed in COS-1 cells | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.1 | - |
CMP-N-acetylneuraminate | apparent value, recombinant enzyme, using pyridylamine-type 2H as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C | Homo sapiens | |
2.4.3.1 | 0.11 | - |
CMP-N-acetylneuraminate | apparent value, recombinant enzyme, using pyridylaminyl-lacto-N-neotetraose as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C | Homo sapiens | |
2.4.3.1 | 0.9 | - |
pyridylaminyl-lacto-N-neotetraose | recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C | Homo sapiens | |
2.4.3.1 | 3.5 | - |
pyridylamine-type 2H | recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 55000 | - |
x * 55000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE | Homo sapiens |
2.4.3.1 | 57000 | - |
x * 57000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.1 | Homo sapiens | P15907 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.3.1 | protein G Sepharose column chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.1 | DLD-1 cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | CMP-N-acetylneuraminate + pyridylamine-type 2H | 2% activity compared to pyridylaminyl-lacto-N-neotetraose | Homo sapiens | CMP + alpha2,6-sialylated blood group type 2H antigen | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + pyridylaminyl-lacto-N-neotetraose | 100% activity | Homo sapiens | CMP + ? | - |
? | |
2.4.3.1 | additional information | the enzyme cannot transfer a N-acetylneuraminate residue to pyridylaminyl-LNFP I, a type 1 chain derivative of the blood group H antigen | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.3.1 | ? | x * 55000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE | Homo sapiens |
2.4.3.1 | ? | x * 57000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.1 | alpha2,6-sialyltransferase | - |
Homo sapiens |
2.4.3.1 | ST6Gal I | - |
Homo sapiens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.4.3.1 | Homo sapiens | the enzyme is up-regulated in human colon cancer | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.3.1 | physiological function | the enzyme is responsible for alpha2,6-sialylated blood group type 2H antigen biosynthesis in human colon cancer cells | Homo sapiens |