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Literature summary extracted from

  • Moen, M.N.; Knaevelsrud, I.; Haugland, G.T.; Grosvik, K.; Birkeland, N.K.; Klungland, A.; Bjelland, S.
    Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxynucleoside triphosphates and ATP/ADP, into short-patch repair (2011), J. Bacteriol., 193, 4495-4508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 overexpression in Escherichia coli Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 25600
-
 x * 25600, SDS-PAGE Thermoplasma acidophilum
3.2.2.27 25601
-
 x * 25601, calculated from sequence Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Thermoplasma acidophilum Q9HKW6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded oligonucleotide + H2O
-
 Thermoplasma acidophilum uracil + double-stranded oligonucleotide with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded oligonucleotide + H2O
-
 Thermoplasma acidophilum uracil + single-stranded oligonucleotide with abasic site
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.27 ? x * 25600, SDS-PAGE Thermoplasma acidophilum
3.2.2.27 ? x * 25601, calculated from sequence Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 Ta0477
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 60
-
 assay at Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.27 additional information
-
 additional information excises 0.04 uracils per sec from uracil-mismatched oligonucleotide Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 7.5
-
 assay at Thermoplasma acidophilum