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Literature summary extracted from

  • Reed, D.W.; Hartzell, P.L.
    The Archaeoglobus fulgidus D-lactate dehydrogenase is a Zn2+ flavoprotein (1999), J. Bacteriol., 181, 7580-7587.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.99.6 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate stimulates Archaeoglobus fulgidus
1.1.99.6 n-dodecyl beta-D-maltoside stimulates Archaeoglobus fulgidus
1.1.99.6 phenazine methosulfate stimulates Archaeoglobus fulgidus
1.1.99.6 Triton X-100 stimulates Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.6 expression as part of a fusion protein with maltose-binding protein in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.6 oxalate 1 mM, addition of Tris buffered oxalate to a reaction mixture inhibits activity Archaeoglobus fulgidus
1.1.99.6 Tris slight inhibition above 30 mM Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.6 0.15
-
 (R)-lactate pH 8.5, 60°C Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.6 Zn2+ the enzyme contains 1 mol of Zn2+ per mol of protein Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.6 Archaeoglobus fulgidus O29853
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.6
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol no activity with L-lactate Archaeoglobus fulgidus pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.6 (R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 Archaeoglobus fulgidus pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 ?
1.1.99.6 additional information methylene blue, dimethylnaphthoquinone, NAD+, and horse heart cytochrome c do not serve as electron acceptors for the purified enzyme Archaeoglobus fulgidus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.6 AF0394
-
 Archaeoglobus fulgidus
1.1.99.6 Dld enzyme
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.6 60
-
 assay at Archaeoglobus fulgidus
1.1.99.6 90
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.99.6 25 95 25°C: 8% of maximal activity, 95°C: about 75% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.6 83
-
 half-life: 105 min Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.6 8
-
 in a 25 mM Tris buffer at 60°C Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.6 7 9.5 pH 7.0: about 30% of maximal activit, pH 9.5: about 55% of maximal activity Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.6 FAD flavoprotein. FAD binds to apoprotein with a 1:1 stoichiometry and is essential for activity. Although the cofactor is tightly associated with the enzyme, it is not covalently attached Archaeoglobus fulgidus