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Literature summary extracted from

  • Haigler, B.; Gibson, D.
    Purification and properties of ferredoxinNAP, a component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816 (1990), J. Bacteriol., 172, 465-468.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.1.7 Iron the protein contains 2 g atoms each of iron Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.7 13600
-
 x * 13600, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.7 Pseudomonas putida Q52126
-
-
1.18.1.7 Pseudomonas putida NCIB 9816 Q52126
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.7 Blue Sepharose column chromatography, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.7 1.52
-
 purified enzyme, in the absence of FAD and with NADPH as cosubstrate, pH and temperature not specified in the publication Pseudomonas putida
1.18.1.7 13.2
-
 purified enzyme, in the absence of FAD and with NADH as cosubstrate, pH and temperature not specified in the publication Pseudomonas putida
1.18.1.7 36.7
-
 purified enzyme, in the presence of FAD and NADPH, pH and temperature not specified in the publication Pseudomonas putida
1.18.1.7 71
-
 purified enzyme, in the presence of FAD and NADH, pH and temperature not specified in the publication Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.7 2 ferricytochrome c + NADH the enzyme has about 2fold higher activity with NADH compared to NADPH Pseudomonas putida 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.18.1.7 2 ferricytochrome c + NADH the enzyme has about 2fold higher activity with NADH compared to NADPH Pseudomonas putida NCIB 9816 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.18.1.7 oxidized cytochrome c + NADPH
-
 Pseudomonas putida reduced cytochrome c + NADP+ + H+
-
 ?
1.18.1.7 oxidized cytochrome c + NADPH
-
 Pseudomonas putida NCIB 9816 reduced cytochrome c + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.7 ? x * 13600, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.18.1.7 NADH-ferredoxinNAP reductase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.7 FAD preferred flavin cofactor Pseudomonas putida
1.18.1.7 NADH NADH is preferred over NADPH Pseudomonas putida
1.18.1.7 NADPH
-
 Pseudomonas putida