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Literature summary extracted from

  • Ozaki, T.; Mishima, S.; Nishiyama, M.; Kuzuyama, T.
    NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids (2009), J. Antibiot., 62, 385-392.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.111 synthesis NovQ is capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids Streptomyces niveus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.111 overexpressed in Escherichia coli as an N-terminal His8-tagged protein Streptomyces niveus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.111 additional information even in the presence of 5mM EDTA, NovQ possessed full enzymatic activity Streptomyces niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.111 0.0151
-
 4-hydroxyphenylpyruvate pH 7.5, 30°C Streptomyces niveus
2.5.1.111 0.0333
-
 dimethylallyl diphosphate pH 7.5, 30°C Streptomyces niveus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.111 additional information magnesium-independent prenyltransferase Streptomyces niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.111 40000
-
 gel filtration Streptomyces niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces niveus
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces niveus 16259
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.111 Streptomyces niveus Q9L9F1
-
-
2.5.1.111 Streptomyces niveus 16259 Q9L9F1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.111
-
 Streptomyces niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 1,6-dihydroxynaphthalene 49.8% yield Streptomyces niveus diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene
-
 ?
2.5.1.111 dimethylallyl diphosphate + 1,6-dihydroxynaphthalene 49.8% yield Streptomyces niveus 16259 diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene
-
 ?
2.5.1.111 dimethylallyl diphosphate + 2,7-dihydroxynaphthalene 31% yield Streptomyces niveus diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene
-
 ?
2.5.1.111 dimethylallyl diphosphate + 2,7-dihydroxynaphthalene 31% yield Streptomyces niveus 16259 diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-coumarate 16.8% yield Streptomyces niveus diphosphate + 4-O-dimethylallylcoumarate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-coumarate 34.4% yield Streptomyces niveus diphosphate + 4-O-dimethylallylcoumarate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-coumarate 16.8% yield Streptomyces niveus 16259 diphosphate + 4-O-dimethylallylcoumarate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces niveus diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces niveus 16259 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + apigenin 7.6% yield Streptomyces niveus diphosphate + 3'-C-dimethylallyl apigenin
-
 ?
2.5.1.111 dimethylallyl diphosphate + caffeate 20% yield Streptomyces niveus diphosphate + 3-O,4-O-bis-(dimethylallyl)caffeate
-
 ?
2.5.1.111 dimethylallyl diphosphate + caffeate 17.3% yield Streptomyces niveus diphosphate + 3-O-(dimethylallyl)caffeate
-
 ?
2.5.1.111 dimethylallyl diphosphate + caffeate 26.1% yield Streptomyces niveus diphosphate + 4-O-(dimethylallyl)caffeate
-
 ?
2.5.1.111 dimethylallyl diphosphate + naringenin 87.2% yield Streptomyces niveus diphosphate + 3'-C-dimethylallyl naringenin
-
 ?
2.5.1.111 dimethylallyl diphosphate + naringenin 11.1% yield Streptomyces niveus diphosphate + 4'-O-dimethylallyl naringenin
-
 ?
2.5.1.111 dimethylallyl diphosphate + resveratrol 12.3% yield Streptomyces niveus diphosphate + 3'-dimethylallyl resveratrol
-
 ?
2.5.1.111 additional information no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene Streptomyces niveus ?
-
 ?
2.5.1.111 additional information no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene Streptomyces niveus 16259 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.111 monomer 1 * 40000, SDS-PAGE Streptomyces niveus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.111 NovQ
-
 Streptomyces niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.111 30
-
 assay at Streptomyces niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.111 0.083
-
 dimethylallyl diphosphate pH 7.5, 30°C Streptomyces niveus
2.5.1.111 0.083
-
 4-hydroxyphenylpyruvate pH 7.5, 30°C Streptomyces niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.111 7.5
-
 assay at Streptomyces niveus