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Literature summary extracted from

  • Mojzita, D.; Koivistoinen, O.M.; Maaheimo, H.; Penttilae, M.; Ruohonen, L.; Richard, P.
    Identification of the galactitol dehydrogenase, LadB, that is part of the oxido-reductive D-galactose catabolic pathway in Aspergillus niger (2012), Fungal Genet. Biol., 49, 152-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.16 gene lad1, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trichoderma reesei
1.1.1.16 gene ladB, orf JGI183900, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression of gene ladB under the lac promoter, functional co-expression of Aspergillus niger wild-type and C-terminally His6-tagged LadB and LadA, encoding L-arabitol dehydrogenae, in Saccharomyces cerevisiae strain CEN.PK2-1D Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.16 additional information construction of ladBDELTA enzyme-deficient Aspergillus niger strains Aspergillus niger
1.1.1.16 additional information construction of ladBDELTA enzyme-deficient Aspergillus niger strains Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.16 1
-
 L-arabitol pH and temperature not specified in the publication Trichoderma reesei
1.1.1.16 55
-
 L-arabitol pH and temperature not specified in the publication Aspergillus niger
1.1.1.16 200
-
 galactitol about, pH and temperature not specified in the publication Aspergillus niger
1.1.1.16 200
-
 galactitol about, pH and temperature not specified in the publication Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.16 galactitol + NAD+ Aspergillus niger
-
 L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+ Trichoderma reesei L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+ Aspergillus niger ATCC 1015, CBS 113.46
-
 L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+ Trichoderma reesei QM6a L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+ Trichoderma reesei Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase L-xylulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+ Trichoderma reesei QM6a Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase L-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.16 Aspergillus niger
-
 gene ladB
-
1.1.1.16 Aspergillus niger ATCC 1015, CBS 113.46
-
 gene ladB
-
1.1.1.16 Trichoderma reesei
-
 gene lad1
-
1.1.1.16 Trichoderma reesei QM6a
-
 gene lad1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.16 recombinant C-terminally His6-tagged LadB from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.16 galactitol + NAD+
-
 Aspergillus niger L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+
-
 Aspergillus niger L-xylo-3-hexulose + NADH + H+ HPLC and NMR product analyses r
1.1.1.16 galactitol + NAD+
-
 Trichoderma reesei L-xylo-3-hexulose + NADH + H+ HPLC and NMR product analyses r
1.1.1.16 galactitol + NAD+ L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei Trichoderma reesei L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+
-
 Aspergillus niger ATCC 1015, CBS 113.46 L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 galactitol + NAD+
-
 Aspergillus niger ATCC 1015, CBS 113.46 L-xylo-3-hexulose + NADH + H+ HPLC and NMR product analyses r
1.1.1.16 galactitol + NAD+
-
 Trichoderma reesei QM6a L-xylo-3-hexulose + NADH + H+ HPLC and NMR product analyses r
1.1.1.16 galactitol + NAD+ L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei Trichoderma reesei QM6a L-xylo-3-hexulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+
-
 Trichoderma reesei L-xylulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+ Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase Trichoderma reesei L-xylulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+
-
 Trichoderma reesei QM6a L-xylulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+ Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase Trichoderma reesei QM6a L-xylulose + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+
-
 Aspergillus niger ? + NADH + H+
-
 r
1.1.1.16 L-arabitol + NAD+
-
 Aspergillus niger ATCC 1015, CBS 113.46 ? + NADH + H+
-
 r
1.1.1.16 additional information the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol Aspergillus niger ?
-
 ?
1.1.1.16 additional information the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol Trichoderma reesei ?
-
 ?
1.1.1.16 additional information the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol Aspergillus niger ATCC 1015, CBS 113.46 ?
-
 ?
1.1.1.16 additional information the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol Trichoderma reesei QM6a ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.16 galactitol dehydrogenase
-
 Aspergillus niger
1.1.1.16 Lad1
-
 Trichoderma reesei
1.1.1.16 LadB
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.16 NAD+ dependent on Aspergillus niger
1.1.1.16 NAD+ dependent on Trichoderma reesei
1.1.1.16 NADH dependent on Aspergillus niger
1.1.1.16 NADH dependent on Trichoderma reesei

Expression

EC Number Organism Comment Expression
1.1.1.16 Trichoderma reesei gene lad1 is induced in response to L-arabinose, D-galactose, and galactitol up
1.1.1.16 Aspergillus niger gene ladB is induced in response to D-galactose and galactitol up

General Information

EC Number General Information Comment Organism
1.1.1.16 evolution LadB belongs to the medium-chain dehydrogenases Aspergillus niger
1.1.1.16 malfunction deletion of ladB in Aspergillus niger results in growth arrest on galactitol and significantly slower growth on D-galactose supplemented with a small amount of D-xylose. D-Galactose alone cannot be utilised by Aspergillus niger and the addition of D-xylose stimulates growth on D-galactose via transcriptional activation of the D-xylose-inducible reductase gene, xyrA Aspergillus niger
1.1.1.16 metabolism LadB catalyzes a step in the oxido-reductive pathway for D-galactose catabolism in filamentous fungi, overview Aspergillus niger