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Literature summary extracted from

  • Wang, P.; Jin, M.; Zhu, G.
    Biochemical and molecular characterization of NAD(+)-dependent isocitrate dehydrogenase from the ethanologenic bacterium Zymomonas mobilis (2012), FEMS Microbiol. Lett., 327, 134-141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 Ca2+
-
 Zymomonas mobilis
1.1.1.41 Co2+
-
 Zymomonas mobilis
1.1.1.41 Cu2+ complete inhibition Zymomonas mobilis
1.1.1.41 Ni2+
-
 Zymomonas mobilis
1.1.1.41 Zn2+ complete inhibition Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.245
-
 NAD+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 0.312
-
 NAD+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 7.7
-
 NADP+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 8.2
-
 NADP+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Co2+ activates, 42.5% activity compared to Mn2+ Zymomonas mobilis
1.1.1.41 Mg2+ activates, 78% activity compared to Mn2+ Zymomonas mobilis
1.1.1.41 Mn2+ activates, best cation Zymomonas mobilis
1.1.1.41 additional information the recombinant ZmIDH activity is completely dependent on the divalent cation and Mn2+ is the most effective cation Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 46000
-
 2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE Zymomonas mobilis
1.1.1.41 74000
-
 recombinant His6-tagged enzyme, gel filtration Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Zymomonas mobilis
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ Zymomonas mobilis ATCC 10988
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Zymomonas mobilis
-
 ssp. mobiliss
-
1.1.1.41 Zymomonas mobilis ATCC 10988
-
 ssp. mobiliss
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Zymomonas mobilis 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ poor performance of the recombinant ZmIDH in decarboxylation Zymomonas mobilis 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+
-
 Zymomonas mobilis ATCC 10988 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ poor performance of the recombinant ZmIDH in decarboxylation Zymomonas mobilis ATCC 10988 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NADP+ poor performance of the recombinant ZmIDH in decarboxylation Zymomonas mobilis 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.41 isocitrate + NADP+ poor performance of the recombinant ZmIDH in decarboxylation Zymomonas mobilis ATCC 10988 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 dimer 2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Zymomonas mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 55
-
-
 Zymomonas mobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.41 25 60 and above, activity range, profile overview Zymomonas mobilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.41 40
-
 purified recombinant His-tagged enzyme, rapid inactivation above Zymomonas mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 14
-
 NADP+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 25
-
 NADP+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 88
-
 NAD+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 112
-
 NAD+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 8
-
 with Mn2+ Zymomonas mobilis
1.1.1.41 8.5
-
 with Mg2+ Zymomonas mobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.41 6.5 10 activity range, profile overview Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ Zymomonas mobilis
1.1.1.41 NADP+ ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ Zymomonas mobilis

General Information

EC Number General Information Comment Organism
1.1.1.41 evolution the recombinant ZmIDH is mainly NAD+-dependent and its catalytic efficiency (kcat/Km) is relative low when compared to the prokaryotic NADP+-IDHs Zymomonas mobilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.41 0.000002
-
 NADP+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 0.000003
-
 NADP+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 0.0003
-
 NAD+ pH 7.5, 37°C, with Mg2+, recombinant enzyme Zymomonas mobilis
1.1.1.41 0.0005
-
 NAD+ pH 7.5, 37°C, with Mn2+, recombinant enzyme Zymomonas mobilis