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Literature summary extracted from

  • Mann, F.M.; Thomas, J.A.; Peters, R.J.
    Rv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis (2011), FEBS Lett., 585, 549-554.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.1 expressed in Escherichia coli strain C41 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.1 0.058
-
 dimethylallyl diphosphate in 50 mM sodium phosphate (pH 7.0), at 37°C Mycobacterium tuberculosis
2.5.1.1 0.163
-
 isopentenyl diphosphate in 50 mM sodium phosphate (pH 7.0), at 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.1 Mycobacterium tuberculosis
-
-
-
2.5.1.1 Mycobacterium tuberculosis CDC 1551
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.1 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.1 dimethylallyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis (E)-geranyl diphosphate + diphosphate
-
 ?
2.5.1.1 dimethylallyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis CDC 1551 (E)-geranyl diphosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.1 (E)-geranyl diphosphate synthase
-
 Mycobacterium tuberculosis
2.5.1.1 Rv0989c
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.1 0.012
-
 isopentenyl diphosphate in 50 mM sodium phosphate (pH 7.0), at 37°C Mycobacterium tuberculosis
2.5.1.1 0.085
-
 dimethylallyl diphosphate in 50 mM sodium phosphate (pH 7.0), at 37°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.5.1.1 metabolism the enzyme is able to provide the geranyl diphosphate precursor required for efficient production of both double bond isomers of farnesyl diphosphate in Mycobacterium tuberculosis Mycobacterium tuberculosis