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Literature summary extracted from

  • Girish, T.S.; Navratna, V.; Gopal, B.
    Structure and nucleotide specificity of Staphylococcus aureus dihydrodipicolinate reductase (DapB) (2011), FEBS Lett., 585, 2561-2567.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.8 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.8 using 2.0 M ammonium sulfate and 0.2 M sodium acetate pH 4.5 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.8 K35A the mutation does not alter the oligomeric status or spectroscopic properties of the enzyme, however, NADPH binding is altered by ca 20fold with much less change (ca 4fold) in NADH binding compared to the wild type enzyme Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.8 Staphylococcus aureus Q5HG24
-
-
1.17.1.8 Staphylococcus aureus COL Q5HG24
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.8 Ni2+-NTA affinity column chromatography and DEAE-Sepharose column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
 Staphylococcus aureus (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
 Staphylococcus aureus COL (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+
-
 Staphylococcus aureus (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+
-
 Staphylococcus aureus COL (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.17.1.8 DapB
-
 Staphylococcus aureus
1.17.1.8 DHDPR
-
 Staphylococcus aureus
1.17.1.8 dihydrodipicolinate reductase
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.8 NADH
-
 Staphylococcus aureus
1.17.1.8 NADPH NADPH is a more preferred co-factor than NADH Staphylococcus aureus