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Literature summary extracted from

  • Kouril, T.; Wieloch, P.; Reimann, J.; Wagner, M.; Zaparty, M.; Albers, S.V.; Schomburg, D.; Ruoff, P.; Siebers, B.
    Unraveling the function of the two Entner-Doudoroff branches in the thermoacidophilic crenarchaeon Sulfolobus solfataricus P2 (2013), FEBS J., 280, 1126-1138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.165 expressed in Escherichia coli BL21(DE3)-RIL cells Saccharolobus solfataricus
2.7.1.165 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 ATP weak substrate inhibition Saccharolobus solfataricus
2.7.1.165 D-glycerate substrate inhibition; substrate inhibition at glycerate concentrations higher than 2 mM at 80°C, above 2 mM at 70°C and 5 mM at 65°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Mg2+ dependent on Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 44400
-
 2 * 44400, calculated from amino acid sequence Saccharolobus solfataricus
2.7.1.165 44400
-
 2 * 44400, calculated from sequence Saccharolobus solfataricus
2.7.1.165 49000
-
 2 * 49000, SDS-PAGE Saccharolobus solfataricus
2.7.1.165 90000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.165 ATP + D-glycerate Saccharolobus solfataricus a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate Saccharolobus solfataricus P2 a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus P2 a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus P2
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Saccharolobus solfataricus Q7LXP1
-
-
2.7.1.165 Saccharolobus solfataricus P2 Q7LXP1
-
-
2.7.1.178 Saccharolobus solfataricus
-
-
-
2.7.1.178 Saccharolobus solfataricus Q97U29
-
-
2.7.1.178 Saccharolobus solfataricus P2
-
-
-
2.7.1.178 Saccharolobus solfataricus P2 Q97U29
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165
-
 Saccharolobus solfataricus
2.7.1.165 UNO Q-12 column chromatography and Superdex 200 gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + D-glycerate specific substrate Saccharolobus solfataricus ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C Saccharolobus solfataricus ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate specific substrate Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.165 ATP + D-glycerate the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus P2 ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Saccharolobus solfataricus P2 ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus P2 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 homodimer 2 * 49000, SDS-PAGE Saccharolobus solfataricus
2.7.1.165 homodimer 2 * 44400, calculated from amino acid sequence Saccharolobus solfataricus
2.7.1.165 homodimer 2 * 44400, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.165 class II glycerate kinase
-
 Saccharolobus solfataricus
2.7.1.165 glycerate kinase
-
 Saccharolobus solfataricus
2.7.1.165 Sso-GK
-
 Saccharolobus solfataricus
2.7.1.165 SSO0666
-
 Saccharolobus solfataricus
2.7.1.178 2-keto-3-deoxygluconate kinase
-
 Saccharolobus solfataricus
2.7.1.178 KDGK
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 80
-
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.165 24.5
-
 D-glycerate in 100 mM HEPES/KOH, pH 7.0, at 37°C Saccharolobus solfataricus
2.7.1.165 24.5
-
 D-glycerate pH 6.5, 80°C, Hill equation Saccharolobus solfataricus
2.7.1.165 28.8
-
 ATP in 100 mM HEPES/KOH, pH 7.0, at 37°C Saccharolobus solfataricus
2.7.1.165 28.8
-
 ATP pH 6.5, 80°C, Michaelis–Menten equation Saccharolobus solfataricus
2.7.1.165 35.1
-
 ATP pH 6.5, 80°C, substrate-inhibition model Saccharolobus solfataricus
2.7.1.165 533.3
-
 D-glycerate pH 6.5, 80°C, kinetic model Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.165 0.1
-
 D-glycerate in 100 mM HEPES/KOH, pH 7.0, at 37°C Saccharolobus solfataricus
2.7.1.165 0.1
-
 D-glycerate pH 6.5, 80°C Saccharolobus solfataricus
2.7.1.165 61
-
 ATP in 100 mM HEPES/KOH, pH 7.0, at 37°C Saccharolobus solfataricus
2.7.1.165 61
-
 ATP pH 6.5, 80°C Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.7.1.45 malfunction deletion of the semiphosphorylative Entner-Doudoroff pathway key enzyme 2-keto-3-deoxygluconate kinase in Sulfolobus solfataricus PBL2025 results in a similar growth phenotype on glucose as substrate compared with the wild-type. In contrast, the mutant shows strongly increased concentrations of nonphosphorylative-Entner-Doudoroff intermediates whereas the hexose and pentose phosphates as well as trehalose are decreased. The results indicate (a) that the nonphosphorylative pathway is able to compensate for the missing semiphosphorylative Entner-Doudoroff branch in glucose catabolism, (b) that in addition to its catabolic function the semiphosphorylative Entner-Doudoroff pathway has an additional although not essential role in providing sugar phosphates for anabolism/gluconeogenesis and (c) that glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus
2.7.1.165 metabolism the enzyme plays a major role in controlling the flux between the glycolytic non-phosphorylative-Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus
2.7.1.178 malfunction a 2-keto-3-deoxygluconate kinase deletion strain reveals no difference in glycolytic growth compared with the reference strain Saccharolobus solfataricus