EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | 2-mercaptoethanol | about 140% activity at 5 mM | Sulfurisphaera tokodaii | |
1.2.1.3 | dithiothreitol | about 150% activity at 5 mM | Sulfurisphaera tokodaii | |
1.2.1.5 | NH4+ | 500 mM, 1.31fold activation | Sulfurisphaera tokodaii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.3 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Sulfurisphaera tokodaii |
1.2.1.5 | - |
Sulfurisphaera tokodaii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.3 | C274A | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
1.2.1.3 | C274S | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
1.2.1.3 | E240A | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
1.2.1.3 | E240S | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
1.2.1.3 | E370Q | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
1.2.1.3 | I168A | the mutation results in no obvious change of kinetics properties toward acetaldehyde and a comparable increase of affinity toward NAD+ | Sulfurisphaera tokodaii |
1.2.1.3 | K165Q | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
1.2.1.3 | N142A | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
EC Number | General Stability | Organism |
---|---|---|
1.2.1.5 | the enzyme is stable to 50 mM PMSF | Sulfurisphaera tokodaii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | H2O2 | - |
Sulfurisphaera tokodaii | |
1.2.1.3 | nitroglycerin | more than half of the original activity retained in the presence of 5.5 mM and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin | Sulfurisphaera tokodaii | |
1.2.1.5 | 2-mercaptoethanol | 10% (v/v), 55% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | acetone | 10% (v/v), 21% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | DMSO | 10% (v/v), 32% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | EDTA | 50 mM, 22% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | ethanediol | 10% (v/v), 60% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | guanidine hydrochloride | 10% (v/v), 42% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | H2O2 | the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol | Sulfurisphaera tokodaii | |
1.2.1.5 | nitroglycerin | more than half of the original activity is retained in the presence of 5.5 mM nitroglycerin and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin. Dithiothreitol and alpha-lipoic acid can also restore the nitroglycerin inactivated dehydrogenation to more than half of the original activity | Sulfurisphaera tokodaii | |
1.2.1.5 | PEG-400 | 10% (v/v), 16% inhibition | Sulfurisphaera tokodaii | |
1.2.1.5 | PMSF | 50 mM, no loss of activity | Sulfurisphaera tokodaii | |
1.2.1.5 | Urea | 500 mM, 44% inhibition | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.82 | - |
NAD+ | with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.84 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 3.81 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 6.12 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 6.38 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 8.47 | - |
NADP+ | with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 8.51 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.84 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 3.81 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 6.12 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 6.38 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 8.51 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | K+ | 130% activity at 20 mM | Sulfurisphaera tokodaii | |
1.2.1.3 | Li+ | 119% activity at 20 mM | Sulfurisphaera tokodaii | |
1.2.1.3 | additional information | the enzyme is not influenced by 20 mM Mg2, Cu2+, Ca2+, Ba2+, Ni2+, Co2+, and Sr2+ | Sulfurisphaera tokodaii | |
1.2.1.3 | Na+ | 122% activity at 20 mM | Sulfurisphaera tokodaii | |
1.2.1.3 | NH4+ | 131% activity at 20 mM | Sulfurisphaera tokodaii | |
1.2.1.5 | K+ | 500 mM, 1.3fold activation | Sulfurisphaera tokodaii | |
1.2.1.5 | Li+ | 500 mM, 107% of the activity compared to activity without metal ions | Sulfurisphaera tokodaii | |
1.2.1.5 | Mg2+ | 20 mM, 107% of the activity compared to activity without metal ions | Sulfurisphaera tokodaii | |
1.2.1.5 | Na+ | 500 mM, 1.2fold activation | Sulfurisphaera tokodaii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 50000 | - |
4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
1.2.1.3 | 51300 | - |
4 * 51300, calculated from amino acid sequence | Sulfurisphaera tokodaii |
1.2.1.3 | 200000 | - |
gel filtration | Sulfurisphaera tokodaii |
1.2.1.5 | 50000 | - |
4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
1.2.1.5 | 51300 | - |
4 * 51300, calculation from sequence | Sulfurisphaera tokodaii |
1.2.1.5 | 200000 | - |
gel filtration | Sulfurisphaera tokodaii |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.2.1.3 | dithiothreitol | the enzyme is stable in dithiothreitol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.3 | Ethanol | the enzyme is stable in ethanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.3 | isopropanol | the enzyme is stable in isopropanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.3 | Methanol | the enzyme is stable in methanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.3 | phenylmethylsulfonyl fluoride | the enzyme is stable in phenylmethylsulfonyl fluoride (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.3 | SDS | the enzyme is stable in SDS (90% of the original activity remaining) | Sulfurisphaera tokodaii |
1.2.1.5 | 2-mercaptoethanol | 10% (v/v), 55% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | Acetone | 10% (v/v), 21% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | DMSO | 10% (v/v),% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | Ethanol | 10% (v/v), 2% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | Ethylene glycol | 10% (v/v), 60% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | guanidine-HCl | 10% (v/v), 42% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | isopropanol | 10% (v/v), 8% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | Methanol | 10% (v/v), 7% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | polyethyleneglycol | PEG-400, 10% (v/v), 16% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | SDS | 10% (v/v), 3% loss of activity | Sulfurisphaera tokodaii |
1.2.1.5 | urea | 500 mM, 44% loss of activity | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.3 | Sulfurisphaera tokodaii | - |
- |
- |
1.2.1.3 | Sulfurisphaera tokodaii 7 | - |
- |
- |
1.2.1.5 | Sulfurisphaera tokodaii | Q976X5 | - |
- |
1.2.1.5 | Sulfurisphaera tokodaii 7 | Q976X5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.3 | Ni-NTA affinity column chromatography | Sulfurisphaera tokodaii |
1.2.1.5 | - |
Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.3 | 1,3-dihydroxyacetone + NAD+ + H2O | 53.93% activity compared to acetaldehyde | Sulfurisphaera tokodaii | ? + NADH + H+ | - |
? | |
1.2.1.3 | 3-nitrobenzaldehyde + NAD+ + H2O | 32.98% activity compared to acetaldehyde | Sulfurisphaera tokodaii | 3-nitrobenzoate + NADH + H+ | - |
? | |
1.2.1.3 | acetaldehyde + NAD+ + H2O | 100% activity with acetaldehyde and NAD+ | Sulfurisphaera tokodaii | acetate + NADH + H+ | - |
? | |
1.2.1.3 | acetaldehyde + NAD+ + H2O | 100% activity with acetaldehyde and NAD+ | Sulfurisphaera tokodaii 7 | acetate + NADH + H+ | - |
? | |
1.2.1.3 | acetaldehyde + NADP+ + H2O | low activity with NADP+ | Sulfurisphaera tokodaii | acetate + NADPH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | 35.5% activity compared to acetaldehyde | Sulfurisphaera tokodaii | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | 35.5% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | butyraldehyde + NAD+ + H2O | 48.15% activity compared to acetaldehyde | Sulfurisphaera tokodaii | butyrate + NADH + H+ | - |
? | |
1.2.1.3 | butyraldehyde + NAD+ + H2O | 48.15% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | butyrate + NADH + H+ | - |
? | |
1.2.1.3 | chloral + NAD+ + H2O | 2.67% activity compared to acetaldehyde | Sulfurisphaera tokodaii | trichloroacetate + NADH + H+ | - |
? | |
1.2.1.3 | D-glyceraldehyde + NAD+ + H2O | 50.25% activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate + NADH + H+ | - |
? | |
1.2.1.3 | DL-glyceraldehyde + NAD+ + H2O | 77.27% activity compared to acetaldehyde | Sulfurisphaera tokodaii | DL-glycerate + NADH + H+ | - |
? | |
1.2.1.3 | DL-glyceraldehyde + NAD+ + H2O | low activity with NADP+ | Sulfurisphaera tokodaii | DL-glycerate + NADH + H+ | - |
? | |
1.2.1.3 | DL-glyceraldehyde 3-phosphate + NAD+ + H2O | 52.7% activity compared to acetaldehyde | Sulfurisphaera tokodaii | DL-glycerate 3-phosphate + NADH + H+ | - |
? | |
1.2.1.3 | formaldehyde + NAD+ + H2O | 7.77% activity compared to acetaldehyde | Sulfurisphaera tokodaii | formate + NADH + H+ | - |
? | |
1.2.1.3 | glycolaldehyde + NAD+ + H2O | 75.43% activity compared to acetaldehyde | Sulfurisphaera tokodaii | glycolate + NADH + H+ | - |
? | |
1.2.1.3 | additional information | no activity with methanol, ethanol and isopropanol | Sulfurisphaera tokodaii | ? | - |
? | |
1.2.1.3 | additional information | no activity with methanol, ethanol and isopropanol | Sulfurisphaera tokodaii 7 | ? | - |
? | |
1.2.1.3 | phenylacetaldehyde + NAD+ + H2O | 4.21% activity compared to acetaldehyde | Sulfurisphaera tokodaii | phenylacetate + NADH + H+ | - |
? | |
1.2.1.3 | propionaldehyde + NAD+ + H2O | 56.22% activity compared to acetaldehyde | Sulfurisphaera tokodaii | propionate + NADH + H+ | - |
? | |
1.2.1.3 | propionaldehyde + NAD+ + H2O | 56.22% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | propionate + NADH + H+ | - |
? | |
1.2.1.5 | 1,3-dihydroxyacetone + NAD+ + H2O | 53.93% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | ? + NADH + H+ | - |
? | |
1.2.1.5 | 3-nitrobenzaldehyde + NAD+ + H2O | 32.98% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | 3-nitrobenzoate + NADH + H+ | - |
? | |
1.2.1.5 | acetaldehyde + NAD+ + H2O | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ | Sulfurisphaera tokodaii | acetate + NADH + H+ | - |
? | |
1.2.1.5 | acetaldehyde + NAD+ + H2O | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ | Sulfurisphaera tokodaii 7 | acetate + NADH + H+ | - |
? | |
1.2.1.5 | acetaldehyde + NADP+ + H2O | - |
Sulfurisphaera tokodaii | acetate + NADPH | - |
? | |
1.2.1.5 | benzaldehyde + NAD+ + H2O | 35.5% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | benzoate + NADH + H+ | - |
? | |
1.2.1.5 | benzaldehyde + NAD+ + H2O | 35.5% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | benzoate + NADH + H+ | - |
? | |
1.2.1.5 | butyraldehyde + NAD+ + H2O | 48.15% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | butyrate + NADH + H+ | - |
? | |
1.2.1.5 | butyraldehyde + NAD+ + H2O | 48.15% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | butyrate + NADH + H+ | - |
? | |
1.2.1.5 | D-glyceraldehyde + NAD+ + H2O | 50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii | D-glycerate + NADH + H+ | - |
? | |
1.2.1.5 | D-glyceraldehyde + NADP+ + H2O | 50.25% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate + NADPH | - |
? | |
1.2.1.5 | D-glyceraldehyde 3-phosphate + NAD+ + H2O | 52.7% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate 3-phosphate + NADH + H+ | - |
? | |
1.2.1.5 | glycolaldehyde + NAD+ + H2O | 75.43% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | glycolate + NADH + H+ | - |
? | |
1.2.1.5 | additional information | no activity with methanol or ethanol | Sulfurisphaera tokodaii | ? | - |
? | |
1.2.1.5 | additional information | no activity with methanol or ethanol | Sulfurisphaera tokodaii 7 | ? | - |
? | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | 56.22% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | propionate + NADH + H+ | - |
? | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | 56.22% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | propionate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.3 | homotetramer | 4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
1.2.1.3 | homotetramer | 4 * 51300, calculated from amino acid sequence | Sulfurisphaera tokodaii |
1.2.1.5 | tetramer | 4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
1.2.1.5 | tetramer | 4 * 51300, calculation from sequence | Sulfurisphaera tokodaii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.3 | ALDH | - |
Sulfurisphaera tokodaii |
1.2.1.5 | ST0064 | - |
Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 80 | - |
- |
Sulfurisphaera tokodaii |
1.2.1.5 | 80 | - |
- |
Sulfurisphaera tokodaii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 70 | 90 | about 50% activity at 70°C, 100% activity at 80°C, about 80% activity at 90°C | Sulfurisphaera tokodaii |
1.2.1.5 | 70 | 90 | 70°C: about 50% of maximal activity, 90°C: about 90% of maximal activity | Sulfurisphaera tokodaii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 70 | 90 | the enzyme shows no obvious loss of activity after heat-treatment at 70°C for 12 h, and more than 60% of the original activity remains after treatment at 80°C for 12 h. The half-life at 90°C is 4 h | Sulfurisphaera tokodaii |
1.2.1.5 | additional information | - |
coenzyme NAD+ or NADP+ can regulate the enzymatic thermostability | Sulfurisphaera tokodaii |
1.2.1.5 | 70 | 80 | about 20% increase of activity after treatment at 70 and 80°C for 2 h, probably due to thermal activation of the enzyme | Sulfurisphaera tokodaii |
1.2.1.5 | 90 | - |
half-life: 4 h. Significant protection against thermal inactivation by 1 mM NAD+ or NADP+ when the preheating time is above 4 h at 90°C | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.6 | - |
NAD+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.83 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.92 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 1.14 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 1.63 | - |
NADP+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 2.05 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 3.71 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.83 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.92 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 1.14 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 2.05 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 3.71 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 8 | - |
- |
Sulfurisphaera tokodaii |
1.2.1.5 | 8 | - |
- |
Sulfurisphaera tokodaii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 6 | 9 | about 60% activity at pH 6.0, about 70% activity at pH 7.0, 100% activity pH 8.0, about 85% activity at pH 9.0 | Sulfurisphaera tokodaii |
1.2.1.5 | 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity | Sulfurisphaera tokodaii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | NAD+ | the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ | Sulfurisphaera tokodaii | |
1.2.1.3 | NADP+ | the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ | Sulfurisphaera tokodaii | |
1.2.1.5 | NAD+ | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii | |
1.2.1.5 | NADP+ | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.11 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.14 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.18 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.19 | - |
NADP+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.73 | - |
NAD+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 0.98 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.3 | 2.45 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.11 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.14 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.18 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 0.98 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.2.1.5 | 2.45 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii |