BRENDA - Enzyme Database

Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea

Timpson, L.M.; Alsafadi, D.; Mac Donnchadha, C.; Liddell, S.; Sharkey, M.A.; Paradisi, F.; Extremophiles 16, 57-66 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.71
overexpression in Escherichia coli as a His-tagged protein
Haloarcula marismortui
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.71
0.029
-
NADPH
pH 6.0, 50°C
Haloarcula marismortui
1.1.1.71
0.101
-
NADP+
pH 10.0, 50°C
Haloarcula marismortui
1.1.1.71
5.1
-
acetaldehyde
pH 6.0, 50°C
Haloarcula marismortui
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.71
K+
optimally active in the presence of 2 M KCl
Haloarcula marismortui
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.71
41600
-
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.1.1.71
acetonitrile
10%, enzyme retains 69% of activity following incubation
Haloarcula marismortui
1.1.1.71
dimethyl sulfoxide
10%, enzyme retains 78% of activity following incubation
Haloarcula marismortui
1.1.1.71
tetrahydrofuran
10%, decativates
Haloarcula marismortui
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.71
Haloarcula marismortui
Q5V676
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.71
-
Haloarcula marismortui
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.71
-20°C, retains approximately 80% of its original activity after 10 days
Haloarcula marismortui
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.71
1-butanol + NADP+
117% of the activity compared to ethanol
722208
Haloarcula marismortui
butanal + NADPH + H+
-
-
-
-
1.1.1.71
1-pentanol + NADP+
74% of the activity compared to ethanol
722208
Haloarcula marismortui
pentanal + NADPH + H+
-
-
-
-
1.1.1.71
1-propanol + NADP+
137% of the activity compared to ethanol
722208
Haloarcula marismortui
propanal + NADPH + H+
-
-
-
-
1.1.1.71
2-butanol + NADP+
43% of the activity compared to ethanol
722208
Haloarcula marismortui
2-butanone + NADPH + H+
-
-
-
-
1.1.1.71
acetaldehyde + NADPH + H+
-
722208
Haloarcula marismortui
ethanol + NADP+
-
-
-
?
1.1.1.71
ethanol + NAD+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADH + H+
-
-
-
?
1.1.1.71
ethanol + NADP+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
additional information
the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates
722208
Haloarcula marismortui
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.71
?
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.71
60
-
-
Haloarcula marismortui
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.71
40
80
40°C: about 50% of maximal activity, 80°C: about 70% of maximal activity
Haloarcula marismortui
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6
-
reductive reaction
Haloarcula marismortui
1.1.1.71
10
-
oxidative reaction
Haloarcula marismortui
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NAD+
activity is 75% less than that detected with NADP+
Haloarcula marismortui
1.1.1.71
NADP+
preferred cofactor. Activity with NAD+ is 75% less than that detected with NADP+
Haloarcula marismortui
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
overexpression in Escherichia coli as a His-tagged protein
Haloarcula marismortui
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NAD+
activity is 75% less than that detected with NADP+
Haloarcula marismortui
1.1.1.71
NADP+
preferred cofactor. Activity with NAD+ is 75% less than that detected with NADP+
Haloarcula marismortui
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.71
0.029
-
NADPH
pH 6.0, 50°C
Haloarcula marismortui
1.1.1.71
0.101
-
NADP+
pH 10.0, 50°C
Haloarcula marismortui
1.1.1.71
5.1
-
acetaldehyde
pH 6.0, 50°C
Haloarcula marismortui
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.71
K+
optimally active in the presence of 2 M KCl
Haloarcula marismortui
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.71
41600
-
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.1.1.71
acetonitrile
10%, enzyme retains 69% of activity following incubation
Haloarcula marismortui
1.1.1.71
dimethyl sulfoxide
10%, enzyme retains 78% of activity following incubation
Haloarcula marismortui
1.1.1.71
tetrahydrofuran
10%, decativates
Haloarcula marismortui
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
-
Haloarcula marismortui
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.71
-20°C, retains approximately 80% of its original activity after 10 days
Haloarcula marismortui
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.71
1-butanol + NADP+
117% of the activity compared to ethanol
722208
Haloarcula marismortui
butanal + NADPH + H+
-
-
-
-
1.1.1.71
1-pentanol + NADP+
74% of the activity compared to ethanol
722208
Haloarcula marismortui
pentanal + NADPH + H+
-
-
-
-
1.1.1.71
1-propanol + NADP+
137% of the activity compared to ethanol
722208
Haloarcula marismortui
propanal + NADPH + H+
-
-
-
-
1.1.1.71
2-butanol + NADP+
43% of the activity compared to ethanol
722208
Haloarcula marismortui
2-butanone + NADPH + H+
-
-
-
-
1.1.1.71
acetaldehyde + NADPH + H+
-
722208
Haloarcula marismortui
ethanol + NADP+
-
-
-
?
1.1.1.71
ethanol + NAD+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADH + H+
-
-
-
?
1.1.1.71
ethanol + NADP+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
additional information
the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates
722208
Haloarcula marismortui
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.71
?
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.71
60
-
-
Haloarcula marismortui
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.71
40
80
40°C: about 50% of maximal activity, 80°C: about 70% of maximal activity
Haloarcula marismortui
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6
-
reductive reaction
Haloarcula marismortui
1.1.1.71
10
-
oxidative reaction
Haloarcula marismortui