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Literature summary extracted from

  • Timpson, L.M.; Alsafadi, D.; Mac Donnchadha, C.; Liddell, S.; Sharkey, M.A.; Paradisi, F.
    Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea (2012), Extremophiles, 16, 57-66.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.71 overexpression in Escherichia coli as a His-tagged protein Haloarcula marismortui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.029
-
NADPH pH 6.0, 50°C Haloarcula marismortui
1.1.1.71 0.101
-
NADP+ pH 10.0, 50°C Haloarcula marismortui
1.1.1.71 5.1
-
acetaldehyde pH 6.0, 50°C Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.71 K+ optimally active in the presence of 2 M KCl Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.71 41600
-
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry Haloarcula marismortui

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.71 acetonitrile 10%, enzyme retains 69% of activity following incubation Haloarcula marismortui
1.1.1.71 dimethyl sulfoxide 10%, enzyme retains 78% of activity following incubation Haloarcula marismortui
1.1.1.71 tetrahydrofuran 10%, decativates Haloarcula marismortui

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Haloarcula marismortui Q5V676
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71
-
Haloarcula marismortui

Storage Stability

EC Number Storage Stability Organism
1.1.1.71 -20°C, retains approximately 80% of its original activity after 10 days Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 1-butanol + NADP+ 117% of the activity compared to ethanol Haloarcula marismortui butanal + NADPH + H+
-
?
1.1.1.71 1-pentanol + NADP+ 74% of the activity compared to ethanol Haloarcula marismortui pentanal + NADPH + H+
-
?
1.1.1.71 1-propanol + NADP+ 137% of the activity compared to ethanol Haloarcula marismortui propanal + NADPH + H+
-
?
1.1.1.71 2-butanol + NADP+ 43% of the activity compared to ethanol Haloarcula marismortui 2-butanone + NADPH + H+
-
?
1.1.1.71 acetaldehyde + NADPH + H+
-
Haloarcula marismortui ethanol + NADP+
-
?
1.1.1.71 ethanol + NAD+ enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+ Haloarcula marismortui acetaldehyde + NADH + H+
-
?
1.1.1.71 ethanol + NADP+ enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+ Haloarcula marismortui acetaldehyde + NADPH + H+
-
?
1.1.1.71 additional information the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates Haloarcula marismortui ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.71 ? x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry Haloarcula marismortui

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 ADH12
-
Haloarcula marismortui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.71 60
-
-
Haloarcula marismortui

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.71 40 80 40°C: about 50% of maximal activity, 80°C: about 70% of maximal activity Haloarcula marismortui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 6
-
reductive reaction Haloarcula marismortui
1.1.1.71 10
-
oxidative reaction Haloarcula marismortui

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NAD+ activity is 75% less than that detected with NADP+ Haloarcula marismortui
1.1.1.71 NADP+ preferred cofactor. Activity with NAD+ is 75% less than that detected with NADP+ Haloarcula marismortui