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Literature summary extracted from

  • Perez-Arellano, I.; Cervera, J.
    Glutamate kinase from Thermotoga maritima: characterization of a thermophilic enzyme for proline biosynthesis (2010), Extremophiles, 14, 409-415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.11 expressed in Escherichia coli as a His-tagged fusion protein Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.11 ADP
-
 Thermotoga maritima
2.7.2.11 cis-4-hydroxy-L-proline
-
 Thermotoga maritima
2.7.2.11 methyl L-proline
-
 Thermotoga maritima
2.7.2.11 trans-4-hydroxy-L-proline
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.11 42000
-
 SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.11 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.11 (NH4)2SO4 precipitation and a single ion-exchange chromatography step are used Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.11 ATP + L-glutamate
-
 Thermotoga maritima ADP + L-glutamate 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.11 tetramer gel filtration, 4 * 42000 Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.2.11 glutamate kinase
-
 Thermotoga maritima
2.7.2.11 proB
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.11 83
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.11 70 85 the enzyme retains approximately 100, 90 and 65% of its activity when heated for 45 min at 70, 80 and 85°C, respectively Thermotoga maritima
2.7.2.11 90
-
 at 90°C, tmGK displays around 40% of the activity when heated for 45 min Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.11 6.5 9
-
 Thermotoga maritima