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Literature summary extracted from

  • Purcarea, C.; Simon, V.; Prieur, D.; Herve, G.
    Purification and characterization of carbamoyl-phosphate synthetase from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi (1996), Eur. J. Biochem., 236, 189-199.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.16 stabilty under pressure, progressive inactivation down to 50% of initial activity at 200 MPa, this residual activity is constant at 200 MPa for at least 50 min Pyrococcus abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 AMP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 CTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 dCTP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 dGTP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 ITP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 TTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 UTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
6.3.4.16 XTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 2.3
-
 CO2 NaHCO3, 37°C, pH 8 Pyrococcus abyssi
6.3.4.16 7.2
-
 CO2 NaHCO3, 70°C, pH 7.5 Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 46600
-
 1 * 46600, SDS-PAGE Pyrococcus abyssi
6.3.4.16 50700
-
 gel filtration Pyrococcus abyssi

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Pyrococcus abyssi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.16
-
 Pyrococcus abyssi

Storage Stability

EC Number Storage Stability Organism
6.3.4.16 4°C, 6 months, stable Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 ATP + NH3 + CO2 + H2O
-
 Pyrococcus abyssi ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 monomer 1 * 46600, SDS-PAGE Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
6.3.4.16 carbamoyl-phosphate synthetase
-
 Pyrococcus abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 95
-
 3 h, 50% loss of activity Pyrococcus abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.16 7.4
-
 70°C Pyrococcus abyssi
6.3.4.16 7.6 8.6 at 37°C Pyrococcus abyssi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.16 5.7 8.5 pH 5.7: about 60% of maximal activit, pH 8.5: about 80% of maximal activity, 70°C Pyrococcus abyssi
6.3.4.16 7 10 pH 7.0: about 60% of maximal activity, pH 10.0: about 60% of maximal activity, 37°C Pyrococcus abyssi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.4.16 Pyrococcus abyssi isoelectric focusing
-
 4.7