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Literature summary extracted from

  • Stournaras, C.; Maurer, P.; Kurz, G.
    6-Phospho-D-gluconate dehydrogenase from Pseudomonas fluorescens. Properties and subunit structure (1983), Eur. J. Biochem., 130, 391-396.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.351 31000
-
 4 * 31000, sedimentation equilibrium analysis Pseudomonas fluorescens
1.1.1.351 32000
-
 4 * 32000, SDS-PAGE Pseudomonas fluorescens
1.1.1.351 115000
-
 catalytically active form, gel filtration Pseudomonas fluorescens
1.1.1.351 126000
-
 native protein, gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.351 Pseudomonas fluorescens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.351 culture condition:D-glucose-grown cell
-
 Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.351 0.023
-
 using NAD+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C Pseudomonas fluorescens
1.1.1.351 0.121
-
 using NADP+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.351 6-phospho-D-gluconate + NAD+
-
 Pseudomonas fluorescens D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+
-
 Pseudomonas fluorescens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.351 homotetramer 4 * 32000, SDS-PAGE Pseudomonas fluorescens
1.1.1.351 homotetramer 4 * 31000, sedimentation equilibrium analysis Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.351 6-phospho-D-gluconate dehydrogenase (decarboxylating)
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.351 8.2
-
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.351 NAD+ the enzyme is active with both NAD+ and NADP+ Pseudomonas fluorescens
1.1.1.351 NADP+ the enzyme is active with both NAD+ and NADP+. The rate of reaction with NADP+ is 20%, of the rate with NAD+ Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.351 Pseudomonas fluorescens isoelectric focusing
-
 4.6