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Literature summary extracted from

  • Li, L.; Eom, H.J.; Park, J.M.; Seo, E.; Ahn, J.E.; Kim, T.J.; Kim, J.H.; Han, N.S.
    Characterization of the major dehydrogenase related to D-lactic acid synthesis in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 (2012), Enzyme Microb. Technol., 51, 274-279.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 gene lhdD or LEUM 1756, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Star Leuconostoc mesenteroides subsp. mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 additional information the enzyme is not inhibited by Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Na+, or urea Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 SDS over 95% inhibition at 1 mM Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 Zn2+ 70% inhibition at 1 mM Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.5
-
 NADH pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 0.58
-
 pyruvate pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 1.96
-
 NAD+ pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 260
-
 (R)-lactate pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Leuconostoc mesenteroides subsp. mesenteroides
-
 pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Leuconostoc mesenteroides subsp. mesenteroides
-
 gene ldhD or LEUM 1756
-
1.1.1.28 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 gene ldhD or LEUM 1756
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28 recombinant His-tagged enzyme 1.82fold from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography Leuconostoc mesenteroides subsp. mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 8.78
-
 purified recombinant enzyme, D-lactate oxidation, pH 11.0, 20°C Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 4450
-
 purified recombinant enzyme, NADH oxidation, pH 8.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 4990
-
 purified recombinant enzyme, pyruvate reduction, pH 8.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Leuconostoc mesenteroides subsp. mesenteroides pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 pyruvate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.28 tetramer
-
 Leuconostoc mesenteroides subsp. mesenteroides

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-(-)-lactate dehydrogenase
-
 Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 LDH
-
 Leuconostoc mesenteroides subsp. mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 20
-
 lactate oxidation reaction Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 30
-
 pyruvate reduction reaction Leuconostoc mesenteroides subsp. mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.28 10 50 activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 2280
-
 (R)-lactate pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 29000
-
 pyruvate pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 8
-
 pyruvate reduction reaction Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 11
-
 lactate oxidation reaction Leuconostoc mesenteroides subsp. mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.28 6 11 pyruvate reduction reaction, activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 7 13 lactate oxidation reaction, activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Leuconostoc mesenteroides subsp. mesenteroides
1.1.1.28 NADH
-
 Leuconostoc mesenteroides subsp. mesenteroides