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Literature summary extracted from

  • Pal, R.; Sanil, N.; Clark, A.
    Developmental studies on the Sigma and Delta-1 glutathione transferases of Lucilia cuprina (2012), Comp. Biochem. Physiol. D, 7, 28-34.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Lucilia cuprina P42860 isozyme GSTD1 or Delta or Theta class isozyme; Sigma and Delta GST isozymes
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 native Sigma and Delta class isozymes by gel filtration, glutathione affinity chromatography, and anion exchange chromatography, 103fold from pupae, 100fold from larvae, 51.7fold from egg (only Delta GST isozyme), and 52fold from adult Lucilia cuprina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 additional information analysis of GST activity and levels of the Sigma and Delta GSTs during development examined at the four developmental stages, egg, larvae, pupae and adult, tissue distribution, overview Lucilia cuprina
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 additional information
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 activity of isozymes from the different stages with other substrates, overview Lucilia cuprina
2.5.1.18 4.33
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 purified overall GST activity in adult, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication Lucilia cuprina
2.5.1.18 4.57
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 purified overall GST activity in eggs, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication Lucilia cuprina
2.5.1.18 6.17
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 purified overall GST activity in larvae, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication Lucilia cuprina
2.5.1.18 14.5
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 purified overall GST activity in pupae, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication Lucilia cuprina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione best substrate Lucilia cuprina S-(2,4-dinitrophenyl)glutathione + HCl
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 ?
2.5.1.18 3,4-dichloronitrobenzene + glutathione
-
 Lucilia cuprina ?
-
 ?
2.5.1.18 4-nitrophenyl acetate + glutathione
-
 Lucilia cuprina ?
-
 ?
2.5.1.18 dehydroascorbic acid + glutathione
-
 Lucilia cuprina ?
-
 ?
2.5.1.18 trans-2-nonenal + glutathione
-
 Lucilia cuprina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 More peptide mapping of Sigma and Delta class isozymes using the sequences of GSTs from Drosophila melanogaster and Musca domestica, overview Lucilia cuprina

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTD1
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 Lucilia cuprina