Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mundt, K.; Wollinsky, B.; Ruan, H.L.; Zhu, T.; Li, S.M.
    Identification of the verruculogen prenyltransferase FtmPT3 by a combination of chemical, bioinformatic and biochemical approaches (2012), ChemBioChem, 13, 2583-2592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.107 expression in Escherichia coli Aspergillus fischeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.107 0.0057
-
 verruculogen pH 7.5, 37°C Aspergillus fischeri
2.5.1.107 0.032
-
 fumitremorgin B pH 7.5, 37°C Aspergillus fischeri
2.5.1.107 0.0615
-
 dimethylallyl diphosphate pH 7.5, 37°C Aspergillus fischeri
2.5.1.107 0.157
-
 12,13-dihydroxyfumitremorgin C pH 7.5, 37°C Aspergillus fischeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.107 45000
-
 5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE Aspergillus fischeri
2.5.1.107 52700
-
 5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE Aspergillus fischeri
2.5.1.107 268000
-
 gel filtration, recombinant protein with His-tag Aspergillus fischeri

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.107 Aspergillus fischeri A1DJ20 putative
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.107 dimethylallyl diphosphate + 12,13-dihydroxyfumitremorgin C
-
 Aspergillus fischeri diphosphate + ?
-
 ?
2.5.1.107 dimethylallyl diphosphate + fumitremorgin B enzyme catalyzes the regular O-prenylation of the hydroxy group at C13 Aspergillus fischeri diphosphate + ?
-
 ?
2.5.1.107 dimethylallyl diphosphate + verruculogen enzyme catalyzes the regular O-prenylation of the hydroxy group at C13 Aspergillus fischeri diphosphate + fumitremorgin A
-
 ?
2.5.1.107 additional information no substrate: fumitremorgin C, which lacks the two hydroxy groups at C12 and C13. No substrates: L-tryptophan, l-tyrosine and diverse derivatives, cyclic dipeptides containing tryptophan and a second amino acid, naringenin, (+)-catechin, 1-naphthol and 1,2-dihydroxynaphthalen Aspergillus fischeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.107 pentamer 5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE Aspergillus fischeri

Synonyms

EC Number Synonyms Comment Organism
2.5.1.107 FtmPT3
-
 Aspergillus fischeri
2.5.1.107 fumitremorgin prenyltransferase 3
-
 Aspergillus fischeri