Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yu, X.; Li, S.M.
    Prenylation of flavonoids by using a dimethylallyltryptophan synthase, 7-DMATS, from Aspergillus fumigatus (2011), ChemBioChem, 12, 2280-2283.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.80 7-DMATS expression in Escherichia coli Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.80 phloretin shows substrate inhibition at 1 mM or higher concentrations Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.80 0.07
-
 Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.13
-
 phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.14
-
 L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus
2.5.1.80 0.16
-
 genistein pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.99
-
 naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 1.1
-
 hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 1.26
-
 eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.80 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.80 Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.80 dimethylallyl diphosphate + apigenin
-
 Aspergillus fumigatus diphosphate + ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + biochanin A 15.9% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + gancaonin A
-
 ?
2.5.1.80 dimethylallyl diphosphate + eriodictyol 19.0% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-(3-methylbut-2-enyl)eriodictyol
-
 ?
2.5.1.80 dimethylallyl diphosphate + genistein 10.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + wighteone
-
 ?
2.5.1.80 dimethylallyl diphosphate + hesperetin 1.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + sigmoidin B
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan L-tryptophan is the preferred substrate Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + luteolin
-
 Aspergillus fumigatus diphosphate + ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + naringenin 1.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-(3-methylbut-2-enyl)naringenin
-
 ?
2.5.1.80 dimethylallyl diphosphate + phloretin 17.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-dimethylallyl-5,7,9,1'-tetrahydroxydihydrochalcone
-
 ?
2.5.1.80 additional information 7-DMATS substrate specificity with flavonoid compounds, overview. Chalcones, isoflavonoids, and flavanones are much better accepted than flavones and flavonols Aspergillus fumigatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.80 7-DMATS
-
 Aspergillus fumigatus
2.5.1.80 dimethylallyltryptophan synthase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.80 37
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.80 0.019
-
 Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.023
-
 naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.026
-
 hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.027
-
 genistein pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.036
-
 phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.23
-
 L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus
2.5.1.80 0.39
-
 eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.5.1.80 evolution the enzyme belongs to the DMATS superfamily, 7-DMATS has a more flexible substrate specificity towards flavonoid compounds than other fungal prenyltransferases Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.80 0.023
-
 naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.024
-
 hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.171
-
 genistein pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.261
-
 Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.286
-
 phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 0.312
-
 eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus
2.5.1.80 1.643
-
 L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus