BRENDA - Enzyme Database

Biosynthesis of the di-meta-hydroxyphenylglycine constituent of the vancomycin-group antibiotic chloroeremomycin

Sandercock, A.M.; Charles, E.H.; Scaife, W.; Kirkpatrick, P.N.; O’Brien, S.W.; Papageorgiou, E.A.; Spencer, J.B.; Williams, D.H.; Chem. Commun. (Camb. ) 2001, 1252-12653 (2001)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.6.1.103
expression of the N-terminal His6-tagged protein in Escherichia coli
Amycolatopsis orientalis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.103
49800
-
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis A82846
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.6.1.103
Amycolatopsis orientalis
-
-
-
2.6.1.103
Amycolatopsis orientalis A82846
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.6.1.103
-
Amycolatopsis orientalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis A82846
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
2.6.1.103
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
2.6.1.103
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis A82846
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.103
?
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Synonyms
EC Number
Synonyms
Commentary
Organism
2.6.1.103
HpgT
-
Amycolatopsis orientalis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.103
30
-
assay at
Amycolatopsis orientalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.103
pyridoxal 5'-phosphate
a pyridoxal-phosphate protein
Amycolatopsis orientalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.103
expression of the N-terminal His6-tagged protein in Escherichia coli
Amycolatopsis orientalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.103
pyridoxal 5'-phosphate
a pyridoxal-phosphate protein
Amycolatopsis orientalis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.103
49800
-
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis A82846
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.103
-
Amycolatopsis orientalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
2.6.1.103
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis A82846
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
2.6.1.103
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
2.6.1.103
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis A82846
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.103
?
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.103
30
-
assay at
Amycolatopsis orientalis
General Information
EC Number
General Information
Commentary
Organism
2.6.1.103
physiological function
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine of the glycopeptide antibiotic chloroeremomycin
Amycolatopsis orientalis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.6.1.103
physiological function
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine of the glycopeptide antibiotic chloroeremomycin
Amycolatopsis orientalis