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Literature summary extracted from

  • Chittori, S.; Savithri, H.S.; Murthy, M.R.
    Structural and mechanistic investigations on Salmonella typhimurium acetate kinase (AckA): identification of a putative ligand binding pocket at the dimeric interface (2012), BMC Struct. Biol., 12, 24.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.1 crystal structures of Salmonella typhimurium AckA unliganded (Form-I, 2.70 A resolution) and citrate-bound (Form-II, 1.90 A resolution) are reported. Unexpectedly, Form-II StAckA structure show a drastic change in the conformation of residues 230-300 compared to that of Form-I Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 2,4-diaminobutyric acid
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 2-oxobutyrate
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 2-oxoglutarate
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 citrate
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 succinate
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.07
-
ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.075
-
ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.078
-
GTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.088
-
CTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.096
-
UTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.1
-
ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.28
-
acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1.2
-
acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 11.2
-
propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 13.5
-
formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 13.5
-
ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Co2+ Vmax: 15%, compared to Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 Mg2+ Vmax: 100% Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 Mn2+ Vmax: 100% Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 Ni2+ Vmax: 5%, compared to Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 45000
-
calculated from cDNA Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Salmonella enterica subsp. enterica serovar Typhimurium P63411
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 using Ni-NTA chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 4°C, 15 days, no loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
r
2.7.2.1 ATP + formate
-
Salmonella enterica subsp. enterica serovar Typhimurium ADP + formyl phosphate
-
?
2.7.2.1 ATP + propionate
-
Salmonella enterica subsp. enterica serovar Typhimurium ADP + propionyl phosphate
-
?
2.7.2.1 CTP + acetate
-
Salmonella enterica subsp. enterica serovar Typhimurium CDP + acetyl phosphate
-
?
2.7.2.1 GTP + acetate
-
Salmonella enterica subsp. enterica serovar Typhimurium GDP + acetyl phosphate
-
?
2.7.2.1 UTP + acetate
-
Salmonella enterica subsp. enterica serovar Typhimurium UDP + acetyl phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.1 StAckA
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 37
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.1 35 37 optimal temperature range Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 870
-
ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 880
-
formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 900
-
ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 940
-
propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1170
-
ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1180
-
acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1750
-
ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1764
-
acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.5
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 7.2 7.5 optimal pH range Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.1 3
-
pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutyrate
2.7.2.1 5
-
pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2,4-diaminobutyric acid
2.7.2.1 7
-
pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoglutarate
2.7.2.1 63
-
pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium citrate
2.7.2.1 75
-
pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium succinate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.1 68
-
formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 85
-
propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 985
-
acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 6300
-
acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 10240
-
ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 11350
-
ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 16860
-
ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 17650
-
ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium