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Literature summary extracted from

  • Morgan, R.E.; Batot, G.O.; Dement, J.M.; Rao, V.A.; Eadsforth, T.C.; Hunter, W.N.
    Crystal structures of Burkholderia cenocepacia dihydropteroate synthase in the apo-form and complexed with the product 7,8-dihydropteroate (2011), BMC Struct. Biol., 11, 21.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.15 medicine DHPS is an antimicrobial therapeutic target by chemical and genetic means Burkholderia cenocepacia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia cenocepacia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.15 purified recombinant apo-enzyme and enzyme in complex with inhibitor sulphadoxine or product 7,8-dihydropteroate, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 7.5 mg/ml protein in 50 mM Tris-HCl, 250 mM NaCl pH 7.5, with 0.001 ml of reservoir solution, containing 0.1 M Tris-HCl, pH 8, 10% PEG 8000, 0.3 M MgCl2, 4°C, X-ray diffraction structure determinationation and analysis at 1.95-2.35 A resolution, molecular replacement and modeling Burkholderia cenocepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 68000
-
 recombinant enzyme, gel filtration Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate Burkholderia cenocepacia
-
 diphosphate + 7,8-dihydropteroate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Burkholderia cenocepacia A0A0H2XP43
-
-
2.5.1.15 no activity in Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography and gel filtration Burkholderia cenocepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate
-
 Burkholderia cenocepacia diphosphate + 7,8-dihydropteroate
-
 r
2.5.1.15 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate
-
 Burkholderia cenocepacia diphosphate + 7,8-dihydropteroate 7,8-dihydropteroate binding mode, overview r

Subunits

EC Number Subunits Comment Organism
2.5.1.15 dimer structure and sequence comparison, overview Burkholderia cenocepacia

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 DHPS
-
 Burkholderia cenocepacia

General Information

EC Number General Information Comment Organism
2.5.1.15 metabolism the enzyme supports the biosynthesis of folate, a key metabolite required to support the synthesis of DNA, and proteins Burkholderia cenocepacia
2.5.1.15 additional information analysis of DHPS active site and interactions with the enzyme product 7,8-dihydropteroate, overview. DHPS shows plasticity near the substrate-binding pocket and a range of loop conformations that contribute to the architecture of the DHPS active site Burkholderia cenocepacia