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Literature summary extracted from

  • Adkins, J.; Jordan, J.; Nielsen, D.
    Engineering Escherichia coli for renewable production of the 5-carbon polyamide building-blocks 5-aminovalerate and glutarate (2013), Biotechnol. Bioeng., 110, 1726-1734.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.48 synthesis production of 5-aminovalerate and glutarate in Escherichia coli. Endogenous over-production of the precursor, lysine, is first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of aspartate kinase III and dihydrodipicolinate synthase. Further disruption of native lysine decarboxylase activity limits cadaverine by-product formation. Co-expression of lysine monooxygenase and 5-aminovaleramide amidohydrolase then results in the production of 0.86 g/l 5-aminovalerate in 48 h. The additional co-expression of glutaric semialdehyde dehydrogenase and 5-aminovalerate aminotransferase leads to the production of 0.82 g/l glutarate under the same conditions. Yields on glucose are 71 and 68 mmol/mol for 5-aminovalerate and glutarate, respectively Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.20 expressed in Escherichia coli BW25113(DE3) cells Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.48 additional information production of 5-aminovalerate and glutarate in Escherichia coli. Endogenous over-production of the precursor, lysine, is first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of aspartate kinase III and dihydrodipicolinate synthase. Further disruption of native lysine decarboxylase activity limits cadaverine by-product formation. Co-expression of lysine monooxygenase and 5-aminovaleramide amidohydrolase then results in the production of 0.86 g/l 5-aminovalerate in 48 h. The additional co-expression of glutaric semialdehyde dehydrogenase and 5-aminovalerate aminotransferase leads to the production of 0.82 g/l glutarate under the same conditions. Yields on glucose are 71 and 68 mmol/mol for 5-aminovalerate and glutarate, respectively Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NADP+ + H2O Pseudomonas putida
-
 glutaric acid + NADPH + H+
-
 ?
1.2.1.20 glutarate semialdehyde + NADP+ + H2O Pseudomonas putida KT 2240
-
 glutaric acid + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.20 Pseudomonas putida
-
-
-
1.2.1.20 Pseudomonas putida KT 2240
-
-
-
2.6.1.48 Pseudomonas putida
-
-
-
2.6.1.48 Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NADP+ + H2O
-
 Pseudomonas putida glutaric acid + NADPH + H+
-
 ?
1.2.1.20 glutarate semialdehyde + NADP+ + H2O
-
 Pseudomonas putida KT 2240 glutaric acid + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.20 davDT davDT
-
 Pseudomonas putida
1.2.1.20 glutarate semialdehyde dehydrogenase
-
 Pseudomonas putida