EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.21 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
2.7.4.24 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.4.21 | - |
Homo sapiens |
2.7.4.24 | - |
Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.21 | K248A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 | Homo sapiens |
2.7.4.21 | R213A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 | Homo sapiens |
2.7.4.24 | K248A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 | Homo sapiens |
2.7.4.24 | R213A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.21 | additional information | isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios | Homo sapiens | |
2.7.4.24 | additional information | isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.21 | 0.00039 | - |
1D-myo-inositol hexakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
2.7.4.21 | 0.037 | - |
ATP | isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.000022 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.00006 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.00011 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.0052 | - |
ADP | isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.022 | - |
ATP | isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.9 | - |
ADP | isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.21 | Homo sapiens | O43314 | - |
- |
2.7.4.24 | Homo sapiens | O43314 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.21 | GSTrap HP column chromatography | Homo sapiens |
2.7.4.24 | GSTrap HP column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.21 | ATP + 1D-myo-inositol hexakisphosphate | - |
Homo sapiens | ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | - |
r | |
2.7.4.24 | ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | - |
Homo sapiens | ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
r | |
2.7.4.24 | ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | - |
Homo sapiens | ATP + 1D-myo-inositol hexakisphosphate | - |
r | |
2.7.4.24 | ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
Homo sapiens | ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.21 | diphosphoinositol pentakisphosphate kinase 2 | - |
Homo sapiens |
2.7.4.21 | PPIP5K2 | isoform | Homo sapiens |
2.7.4.24 | diphosphoinositol pentakisphosphate kinase 2 | - |
Homo sapiens |
2.7.4.24 | PPIP5K2 | isoform | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.21 | 0.03 | - |
1D-myo-inositol hexakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.002 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.13 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.19 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.21 | 0.79 | - |
ATP | isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.21 | 76 | - |
1D-myo-inositol hexakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.037 | - |
ADP | isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 0.21 | - |
ADP | isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 5.9 | - |
ATP | isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 17 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
2.7.4.24 | 2200 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
2.7.4.24 | 8500 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |