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Literature summary extracted from

  • Weaver, J.D.; Wang, H.; Shears, S.B.
    The kinetic properties of a human PPIP5K reveal that its kinase activities are protected against the consequences of a deteriorating cellular bioenergetic environment (2013), Biosci. Rep., 33, e00022.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.21 expressed in Escherichia coli BL21(DE3) cells Homo sapiens
2.7.4.24 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.21
-
Homo sapiens
2.7.4.24
-
Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.21 K248A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 Homo sapiens
2.7.4.21 R213A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 Homo sapiens
2.7.4.24 K248A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 Homo sapiens
2.7.4.24 R213A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.21 additional information isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios Homo sapiens
2.7.4.24 additional information isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.21 0.00039
-
1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C Homo sapiens
2.7.4.21 0.037
-
ATP isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.000022
-
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.00006
-
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.00011
-
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C Homo sapiens
2.7.4.24 0.0052
-
ADP isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.022
-
ATP isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.9
-
ADP isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.21 Homo sapiens O43314
-
-
2.7.4.24 Homo sapiens O43314
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.21 GSTrap HP column chromatography Homo sapiens
2.7.4.24 GSTrap HP column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.21 ATP + 1D-myo-inositol hexakisphosphate
-
Homo sapiens ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate
-
r
2.7.4.24 ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate
-
Homo sapiens ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
r
2.7.4.24 ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate
-
Homo sapiens ATP + 1D-myo-inositol hexakisphosphate
-
r
2.7.4.24 ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
Homo sapiens ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.4.21 diphosphoinositol pentakisphosphate kinase 2
-
Homo sapiens
2.7.4.21 PPIP5K2 isoform Homo sapiens
2.7.4.24 diphosphoinositol pentakisphosphate kinase 2
-
Homo sapiens
2.7.4.24 PPIP5K2 isoform Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.21 0.03
-
1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.002
-
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C Homo sapiens
2.7.4.24 0.13
-
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.19
-
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.21 0.79
-
ATP isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.21 76
-
1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.037
-
ADP isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 0.21
-
ADP isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 5.9
-
ATP isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 17
-
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C Homo sapiens
2.7.4.24 2200
-
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
2.7.4.24 8500
-
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens