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Literature summary extracted from

  • Yu, S.; Jiang, H.; Jiang, B.; Mu, W.
    Characterization of D-lactate dehydrogenase producing D-3-phenyllactic acid from Pediococcus pentosaceus (2012), Biosci. Biotechnol. Biochem., 76, 853-855.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 Ba2+ slight inhibition at 1 mM Pediococcus pentosaceus
1.1.1.28 Cu2+ slight inhibition at 1 mM Pediococcus pentosaceus
1.1.1.28 Mn2+ slight inhibition at 1 mM Pediococcus pentosaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.49
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
1.1.1.28 1.73
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.28 Co2+ activates by 15% at 1 mM Pediococcus pentosaceus
1.1.1.28 Mg2+ very slightly activating Pediococcus pentosaceus
1.1.1.28 Ni2+ activates by 35% at 1 mM Pediococcus pentosaceus
1.1.1.28 Zn2+ very slightly activating Pediococcus pentosaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 37203
-
 x * 37203, sequence calculation Pediococcus pentosaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Pediococcus pentosaceus
-
 pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ Pediococcus pentosaceus ATCC 25745
-
 pyruvate + NADH + H+
-
 r
1.1.1.28 phenylpyruvate + NADH + H+ Pediococcus pentosaceus
-
 (R)-3-phenyllactate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+ Pediococcus pentosaceus ATCC 25745
-
 (R)-3-phenyllactate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Pediococcus pentosaceus
-
-
-
1.1.1.28 Pediococcus pentosaceus ATCC 25745
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 116
-
 substrate phenylpyruvate, pH 5.5, 45°C Pediococcus pentosaceus
1.1.1.28 835
-
 substrate pyruvate, pH 5.5, 45°C Pediococcus pentosaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Pediococcus pentosaceus pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Pediococcus pentosaceus ATCC 25745 pyruvate + NADH + H+
-
 r
1.1.1.28 phenylpyruvate + NADH + H+
-
 Pediococcus pentosaceus (R)-3-phenyllactate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+
-
 Pediococcus pentosaceus ATCC 25745 (R)-3-phenyllactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 ? x * 37203, sequence calculation Pediococcus pentosaceus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-LDH
-
 Pediococcus pentosaceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 45
-
-
 Pediococcus pentosaceus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.28 25 60 activity range, profile overview Pediococcus pentosaceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 30 45 87% activity remaining after 4 h at 30°C, higher loss of activity above 45°C Pediococcus pentosaceus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 173
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
1.1.1.28 320
-
 pyruvate pH 5.5, 45°C Pediococcus pentosaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 5.5
-
-
 Pediococcus pentosaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.28 3 9 activity range, profile overview Pediococcus pentosaceus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Pediococcus pentosaceus
1.1.1.28 NADH
-
 Pediococcus pentosaceus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.28 100
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
1.1.1.28 658
-
 pyruvate pH 5.5, 45°C Pediococcus pentosaceus