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Literature summary extracted from

  • Kawano, S.; Yano, M.; Hasegawa, J.; Yasohara, Y.
    Purification and characterization of an NADH-dependent alcohol dehydrogenase from Candida maris for the synthesis of optically active 1-(pyridyl)ethanol derivatives (2011), Biosci. Biotechnol. Biochem., 75, 1055-1060.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 CoCl2 15% inhibition at 1 mM [Candida] maris
1.1.1.1 HgCl2 complete inhibition at 1 mM [Candida] maris
1.1.1.1 iodoacetate 20% inhibition at 1 mM [Candida] maris
1.1.1.1 MgSO4 19% inhibition at 1 mM [Candida] maris
1.1.1.1 MnCl2 24% inhibition at 1 mM [Candida] maris
1.1.1.1 additional information no inhibition by 4-chloromercuribenzoate at 0.1 mM and by CuSO4 at 1 mM, poor effects by EDTA, 1,20-phenanthroline, 2-mercaptoethanol, and DTT [Candida] maris
1.1.1.1 NEM 17% inhibition at 1 mM [Candida] maris
1.1.1.1 quercetin 22% inhibition at 0.01 mM [Candida] maris
1.1.1.1 ZnSO4 26% inhibition at 1 mM [Candida] maris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 28900
-
 2 * 28900, SDS-PAGE [Candida] maris
1.1.1.1 59900
-
 gel filtration [Candida] maris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 [Candida] maris
-
-
-
1.1.1.1 [Candida] maris IFO10003
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 native (R)-specific alcohol dehydrogenase 37fold from strain IFO10003, by ammonium sulfate fractionation, and anion exchange and hydrophobic interaction chromatography to homogeneity [Candida] maris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 27.6
-
 purified native enzyme, pH 6.5, 30°C [Candida] maris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-butanal + NADH + H+
-
 [Candida] maris 1-butanol + NAD+
-
 r
1.1.1.1 1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
 r
1.1.1.1 1-hexanal + NADH + H+
-
 [Candida] maris 1-hexanol + NAD+
-
 r
1.1.1.1 2,3'-dichloroacetophenone + NADH + H+
-
 [Candida] maris 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 r
1.1.1.1 2,3'-dichloroacetophenone + NADH + H+
-
 [Candida] maris IFO10003 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 r
1.1.1.1 2-acetylcyclohexanone + NADH + H+
-
 [Candida] maris 2-acetylcyclohexanol + NAD+
-
 r
1.1.1.1 2-acetylcyclopentanone + NADH + H+
-
 [Candida] maris 2-acetylcyclopentanol + NAD+
-
 r
1.1.1.1 2-acetylfuran + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 2-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(2-pyridyl)ethanol + NAD+
-
 r
1.1.1.1 2-acetylpyrrole + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 2-acetylthiazole + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 2-acetylthiophene + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 2-butanone + NADH + H+
-
 [Candida] maris 2-butanol + NAD+
-
 r
1.1.1.1 2-chloroacetophenone + NADH + H+ low activity [Candida] maris 1-(2-chlorophenyl)ethanol + NAD+
-
 r
1.1.1.1 2-hexanone + NADH + H+
-
 [Candida] maris 2-hexanol + NAD+
-
 r
1.1.1.1 2-nitrobenzaldehyde + NADH + H+ low activity [Candida] maris 2-nitrobenzyl alcohol + NAD+
-
 r
1.1.1.1 2-octanone + NADH + H+
-
 [Candida] maris 2-octanol + NAD+
-
 r
1.1.1.1 2-oxopentanoate + NADH + H+ low activity [Candida] maris 2-hydroxypentanoate + NAD+
-
 r
1.1.1.1 2-pentanone + NADH + H+
-
 [Candida] maris 2-pentanol + NAD+
-
 r
1.1.1.1 3-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(3-pyridyl)ethanol + NAD+
-
 r
1.1.1.1 3-chlorobenzaldehyde + NADH + H+
-
 [Candida] maris 3-chlorobenzyl alcohol + NAD+
-
 r
1.1.1.1 3-methylbutan-2-one + NADH + H+
-
 [Candida] maris 3-methyl-2-butanol + NAD+
-
 r
1.1.1.1 3-nitroacetophenone + NADH + H+
-
 [Candida] maris 1-(3-nitrophenyl)ethanol + NAD+
-
 r
1.1.1.1 3-nitrobenzaldehyde + NADH + H+
-
 [Candida] maris 3-nitrobenzyl alcohol + NAD+
-
 r
1.1.1.1 4-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(4-pyridyl)ethanol + NAD+
-
 r
1.1.1.1 4-chloroacetophenone + NADH + H+
-
 [Candida] maris 1-(4-chlorophenyl)ethanol + NAD+
-
 r
1.1.1.1 4-chlorobenzaldehyde + NADH + H+
-
 [Candida] maris 4-chlorobenzyl alcohol + NAD+
-
 r
1.1.1.1 4-fluoroacetophenone + NADH + H+
-
 [Candida] maris 1-(4-fluorophenyl)ethanol + NAD+
-
 r
1.1.1.1 4-methylpentan-2-one + NADH + H+
-
 [Candida] maris 4-methyl-2-pentanol + NAD+
-
 r
1.1.1.1 4-nitrobenzaldehyde + NADH + H+
-
 [Candida] maris 4-nitrobenzyl alcohol + NAD+
-
 r
1.1.1.1 5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+
-
 r
1.1.1.1 5-acetylfuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 [Candida] maris ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 [Candida] maris IFO10003 ethanol + NAD+
-
 r
1.1.1.1 acetoin + NADH + H+
-
 [Candida] maris 3-hydroxy-2-butanol + NAD+
-
 r
1.1.1.1 acetone + NADH + H+
-
 [Candida] maris 2-propanol + NAD+
-
 ?
1.1.1.1 acetophenone + NADH + H+
-
 [Candida] maris 1-phenylethanol + NAD+
-
 r
1.1.1.1 acetophenone + NADH + H+
-
 [Candida] maris IFO10003 1-phenylethanol + NAD+
-
 r
1.1.1.1 acetylacetone + 2 NADH + 2 H+
-
 [Candida] maris 2,4-pentanediol + 2 NAD+
-
 r
1.1.1.1 acetylpyrazine + NADH + H+
-
 [Candida] maris (R)-1-(pyrazyl)ethanol + NAD+
-
 r
1.1.1.1 benzaldehyde + NADH + H+
-
 [Candida] maris benzyl alcohol + NAD+
-
 r
1.1.1.1 benzaldehyde + NADH + H+
-
 [Candida] maris IFO10003 benzyl alcohol + NAD+
-
 r
1.1.1.1 benzylacetone + NADH + H+
-
 [Candida] maris 4-phenylbutan-2-ol + NAD+
-
 r
1.1.1.1 chloroacetone + NADH + H+
-
 [Candida] maris 1-chloro-2-propanol + NAD+
-
 r
1.1.1.1 diacetyl + 2 NADH + 2 H+
-
 [Candida] maris 2,3-butandiol + 2 NAD+
-
 r
1.1.1.1 diethylketone + NADH + H+
-
 [Candida] maris 3-pentanol + NAD+
-
 r
1.1.1.1 ethyl 4-chloroacetoacetate + NADH + H+
-
 [Candida] maris ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
 r
1.1.1.1 ethyl acetoacetate + NADH + H+
-
 [Candida] maris ethyl 3-hydroxybutanoate + NAD+
-
 r
1.1.1.1 ethyl pyruvate + NADH + H+
-
 [Candida] maris ethyl lactate + NAD+
-
 r
1.1.1.1 methyl acetoacetate + NADH + H+
-
 [Candida] maris methyl 3-hydroxybutanoate + NAD+
-
 r
1.1.1.1 methyl pyruvate + NADH + H+
-
 [Candida] maris methyl lactate + NAD+
-
 r
1.1.1.1 additional information substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol [Candida] maris ?
-
 ?
1.1.1.1 additional information substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol [Candida] maris IFO10003 ?
-
 ?
1.1.1.1 oxaloacetate + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 propionaldehyde + NADH + H+
-
 [Candida] maris 1-propanol + NAD+
-
 r
1.1.1.1 pyridine 2-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 pyridine 3-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
1.1.1.1 pyridine 4-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 homodimer 2 * 28900, SDS-PAGE [Candida] maris

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 (R)-specific alcohol dehydrogenase
-
 [Candida] maris
1.1.1.1 AFPDH
-
 [Candida] maris
1.1.1.1 NADH-dependent alcohol dehydrogenase
-
 [Candida] maris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 30
-
 assay at [Candida] maris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 10 75 activity range, profile overview [Candida] maris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 25
-
 purified native enzyme, stable up to [Candida] maris
1.1.1.1 40
-
 purified enzyme, inactivation [Candida] maris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 5.5 6 reduction reaction [Candida] maris
1.1.1.1 8.5
-
 oxidation reaction [Candida] maris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 4 8.5 oxidation reaction activity range, profile overview [Candida] maris
1.1.1.1 5.5 9 reduction reaction activity range, profile overview [Candida] maris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 [Candida] maris
1.1.1.1 NADH required [Candida] maris