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Literature summary extracted from
- Reaction mechanism of a new glycosyltrehalose-producing enzyme isolated from the hyperthermophilic archaeum, Sulfolobus solfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 921-924.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.15 | CuSO4 | 5 mM | Saccharolobus solfataricus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 1.2 | - |
maltoheptaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 2.7 | - |
maltohexaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 5 | - |
maltopentaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 21.8 | - |
maltotetraose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 228 | - |
maltotriose | pH 5.5, 60°C | Saccharolobus solfataricus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.15 | Saccharolobus solfataricus | - |
- |
- |
| 5.4.99.15 | Saccharolobus solfataricus KM1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | - |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | maltoheptaose | 88.6% of the activity compared to maltopentaose | Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
| 5.4.99.15 | maltoheptaose | 88.6% of the activity compared to maltopentaose | Saccharolobus solfataricus KM1 | alpha-maltopentaosyl trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | 80% of the activity compared to maltopentaose | Saccharolobus solfataricus | alpha-maltotetraosyltrehalose | - |
? | |
| 5.4.99.15 | maltohexaose | 80% of the activity compared to maltopentaose | Saccharolobus solfataricus KM1 | alpha-maltotetraosyltrehalose | - |
? | |
| 5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus | alpha-maltotriosyltrehalose | - |
? | |
| 5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotriosyltrehalose | - |
? | |
| 5.4.99.15 | maltotetraose | 46.3% of the activity compared to maltopentaose | Saccharolobus solfataricus | alpha-maltosyltrehalose | - |
? | |
| 5.4.99.15 | maltotetraose | 46.3% of the activity compared to maltopentaose | Saccharolobus solfataricus KM1 | alpha-maltosyltrehalose | - |
? | |
| 5.4.99.15 | maltotriose | 5.0% of the activity compared to maltopentaose | Saccharolobus solfataricus | alpha-glucosyltrehalose | - |
? | |
| 5.4.99.15 | maltotriose | 5.0% of the activity compared to maltopentaose | Saccharolobus solfataricus KM1 | alpha-glucosyltrehalose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 60 | - |
assay at | Saccharolobus solfataricus |
| 5.4.99.15 | 70 | 80 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 85 | - |
6 h, 9% loss of activity | Saccharolobus solfataricus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 5 | 6 | - |
Saccharolobus solfataricus |
| 5.4.99.15 | 5.5 | - |
assay at | Saccharolobus solfataricus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 4 | 10 | stable | Saccharolobus solfataricus |
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