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Literature summary extracted from

  • Napoli, J.L.
    Physiological insights into all-trans-retinoic acid biosynthesis (2012), Biochim. Biophys. Acta, 1821, 152-167.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.105 cloning of Rdh7 Rattus norvegicus
1.1.1.105 expression of His6-tagged RDH10 in Spodoptera frugiperda Sf9 insect cell membranes Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.105 L3R/L5R/R16Q/R19Q/R21Q RDH1 mutant, deleting the positive charges from the C-terminal end of the leader, and inserting two arginine residues near the N-terminus of the signaling sequence causes 95% inversion from cytoplasmic to luminal, the mutant faces the lumen Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.105 additional information N-ethylmaleimide does not affect the catalytic action of RDH1, but interferes with its approach to holo-CRBP1, interrupting retinol transfer. Modifying RDH with N-ethylmaleimide inhibited holo-CRBP1-supported generation of retinal by 90% Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.105 additional information
-
 additional information possible Michaelis-Menten relationship between RDH10 and holo-CRBP1 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.105 cytosol
-
 Homo sapiens 5829
-
1.1.1.105 cytosol
-
 Rattus norvegicus 5829
-
1.1.1.105 endoplasmic reticulum membrane cytoplasmic or lumenal facing, RDH1. An N-terminal signaling sequence of 22 residues anchors RDH1 in the endoplasmic reticulum projecting into the cytoplasm Mus musculus 5789
-
1.1.1.105 microsome 80-94% of all enzyme activity Mus musculus
-
-
1.1.1.105 microsome 80-94% of all enzyme activity Homo sapiens
-
-
1.1.1.105 microsome 80-94% of all enzyme activity Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ Mus musculus
-
 all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ Homo sapiens
-
 all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ Rattus norvegicus
-
 all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Homo sapiens
-
-
-
1.1.1.105 Mus musculus
-
-
-
1.1.1.105 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.105 RDH1 activity during purification is maintained only with phosphotidylcholine Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+
-
 Mus musculus all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+
-
 Homo sapiens all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+
-
 Rattus norvegicus all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ RDH interact with/or recognize holo-CRBP1 Homo sapiens all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ RDH interact with/or recognize holo-CRBP1 Rattus norvegicus all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ RDH interact with/or recognize holo-CRBP1, except for RDH10 that does not access holo-CRBP1 as substrate Mus musculus all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 DHRS9
-
 Mus musculus
1.1.1.105 DHRS9
-
 Homo sapiens
1.1.1.105 DHRS9
-
 Rattus norvegicus
1.1.1.105 Rdh1
-
 Mus musculus
1.1.1.105 RDH10
-
 Mus musculus
1.1.1.105 RDH10
-
 Homo sapiens
1.1.1.105 RDH10
-
 Rattus norvegicus
1.1.1.105 RDH16
-
 Homo sapiens
1.1.1.105 RDH2
-
 Rattus norvegicus
1.1.1.105 RDH7
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 NAD+
-
 Mus musculus
1.1.1.105 NAD+
-
 Homo sapiens
1.1.1.105 NAD+
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.1.1.105 evolution RDH1, RDH10 and DHRS9 are microsomal members of the SDR gene family Mus musculus
1.1.1.105 malfunction deleting the positive charges from the C-terminal end of the leader, and inserting two arginine residues near the N-terminus of the signaling sequence causes 95% inversion from cytoplasmic to luminal: i.e. the mutant L3R/L5R/R16Q/R19Q/R21Q faces the lumen Mus musculus
1.1.1.105 metabolism the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview Mus musculus
1.1.1.105 metabolism the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview Homo sapiens
1.1.1.105 metabolism the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview Rattus norvegicus
1.1.1.105 additional information N-ethylmaleimide does not affect the catalytic action of RDH1, but interferes with its approach to holo-CRBP1, interrupting retinol transfer. The membrane context of RDH affects function Mus musculus
1.1.1.105 physiological function RDH1 contributes to a reconstituted pathway of all-trans-retinal biosynthesis, when expressed in intact cells with each of the three retinal dehydrogenases Mus musculus