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Literature summary extracted from

  • Allison, T.M.; Yeoman, J.A.; Hutton, R.D.; Cochrane, F.C.; Jameson, G.B.; Parker, E.J.
    Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans (2010), Biochim. Biophys. Acta, 1804, 1526-1536.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 C21N complete loss of activity Acidithiobacillus ferrooxidans
2.5.1.55 D243A mutation of the absolutely conserved 243AspGlyPro245 motif, complete loss of activity Acidithiobacillus ferrooxidans
2.5.1.55 D243Q mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency Acidithiobacillus ferrooxidans
2.5.1.55 P245A mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.0052
-
phosphoenolpyruvate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 0.012
-
phosphoenolpyruvate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 0.013
-
phosphoenolpyruvate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 0.021
-
D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 0.024
-
D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 2.63
-
D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Cd2+ presence of Cd2+ restores activity to metal-free enzyme, Cd2+ significantly enhances the stability of the enzyme and raises the Tm by 14°C Acidithiobacillus ferrooxidans
2.5.1.55 Co2+ presence of Co2+ restores activity to metal-free enzyme Acidithiobacillus ferrooxidans
2.5.1.55 Mn2+ presence of Mn2+ restores activity to metal-free enzyme Acidithiobacillus ferrooxidans
2.5.1.55 additional information divalent metal ion required, with Mn2+, Co2+ and Cd2+ in decreasing order being able to restore activity to metal-free enzyme Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Acidithiobacillus ferrooxidans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 additional information D-glucose 6-phosphate and D-erythrose 4-phosphate are no substrates Acidithiobacillus ferrooxidans ?
-
?
2.5.1.55 phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate 2-deoxy-D-ribose 5-phosphate is a poor substrate Acidithiobacillus ferrooxidans ? + phosphate
-
?
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
Acidithiobacillus ferrooxidans 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 68
-
wild-type, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 68
-
mutant C21N, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 68
-
mutant D243E, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 69
-
mutant C21N, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 71
-
mutant P245A, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 78
-
mutant D243E, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 79
-
mutant P245A, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
2.5.1.55 82
-
wild-type, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 4.4
-
D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 4.8
-
D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 4.8
-
D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.55 1.8
-
D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 180
-
D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 230
-
D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 370
-
phosphoenolpyruvate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 400
-
phosphoenolpyruvate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.5.1.55 850
-
phosphoenolpyruvate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans