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Literature summary extracted from

  • But, S.Y.; Rozova, O.N.; Khmelenina, V.N.; Reshetnikov, A.S.; Trotsenko, Y.A.
    Properties of recombinant ATP-dependent fructokinase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2012), Biochemistry, 77, 372-377.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.4 expressed in Escherichia coli as a His-tagged fusion protein Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 ADP
-
Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.26
-
D-fructose pH 9, 30°C Methylotuvimicrobium alcaliphilum
2.7.1.4 1.3
-
ATP pH 9, 30°C Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Mn2+ required for optimal activity Methylotuvimicrobium alcaliphilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Methylotuvimicrobium alcaliphilum G4T023
-
-
2.7.1.4 Methylotuvimicrobium alcaliphilum DSM 19304 G4T023
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4 using metal chelate affinity chromatography Methylotuvimicrobium alcaliphilum

Storage Stability

EC Number Storage Stability Organism
2.7.1.4 4°C, 0.2 mM imidazole buffer (pH 8.0), two months Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
Methylotuvimicrobium alcaliphilum ADP + D-fructose 6-phosphate
-
?
2.7.1.4 ATP + D-fructose
-
Methylotuvimicrobium alcaliphilum DSM 19304 ADP + D-fructose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.4 FRK
-
Methylotuvimicrobium alcaliphilum
2.7.1.4 MEALZv4_0616
-
Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 60
-
-
Methylotuvimicrobium alcaliphilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.4 40
-
after 1 h the residual activity is 80% Methylotuvimicrobium alcaliphilum
2.7.1.4 50
-
after 5 min the residual activity is 20% Methylotuvimicrobium alcaliphilum
2.7.1.4 60
-
after 30 min the enzymatic activity is completely lost Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 9
-
-
Methylotuvimicrobium alcaliphilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.4 6.5 9
-
Methylotuvimicrobium alcaliphilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.4 2.5
-
ADP pH 9, 30°C Methylotuvimicrobium alcaliphilum