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Literature summary extracted from

  • Pacholec, M.; Tao, J.; Walsh, C.T.
    CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis (2005), Biochemistry, 44, 14969-14976.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.284 expression in Escherichia coli Streptomyces niveus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.284 additional information no substrate inhibition is observed at 2 mM S-adenosyl-L-methionine Streptomyces niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.284 0.0265
-
8-demethylnovobiocic acid pH 8.5, temperature not specified in the publication Streptomyces niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.284 26500
-
-
Streptomyces niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.284 S-adenosyl-L-methionine + 8-demethylnovobiocic acid Streptomyces niveus the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin S-adenosyl-L-homocysteine + novobiocic acid
-
?
6.3.1.15 ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin Streptomyces niveus the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin AMP + diphosphate + 8-demethylnovobiocic acid
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.284 Streptomyces niveus
-
-
-
6.3.1.15 Streptomyces niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.284
-
Streptomyces niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.284 S-adenosyl-L-methionine + 8-demethylnovobiocic acid
-
Streptomyces niveus S-adenosyl-L-homocysteine + novobiocic acid
-
?
2.1.1.284 S-adenosyl-L-methionine + 8-demethylnovobiocic acid the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus S-adenosyl-L-homocysteine + novobiocic acid
-
?
6.3.1.15 ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin
-
Streptomyces niveus AMP + diphosphate + 8-demethylnovobiocic acid
-
?
6.3.1.15 ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin Streptomyces niveus AMP + diphosphate + 8-demethylnovobiocic acid
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.284 NovO
-
Streptomyces niveus
6.3.1.15 NovL
-
Streptomyces niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.284 0.5
-
8-demethylnovobiocic acid pH 8.5, temperature not specified in the publication Streptomyces niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.284 8.5
-
Tris-HCl buffer Streptomyces niveus

General Information

EC Number General Information Comment Organism
2.1.1.284 physiological function the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus
6.3.1.15 physiological function the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin Streptomyces niveus