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Literature summary extracted from
- Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides (2001), Biochemistry, 40, 4745-4755.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.310 | expression in Escherichia coli as a hexahistidine-tagged protein | Amycolatopsis orientalis |
| 2.4.1.322 | expression in Escherichia coli | Amycolatopsis orientalis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.310 | vancomycin aglycone | severe substrate inhibition above 0.6 mM | Amycolatopsis orientalis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.310 | 0.25 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis | |
| 2.4.1.310 | 1.3 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.310 | 44000 | - |
x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE | Amycolatopsis orientalis |
| 2.4.1.322 | 44000 | - |
x * 44000, SDS-PAGE | Amycolatopsis orientalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis | the enzyme is involved in the biosynthesis of chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis A82846 | the enzyme is involved in the biosynthesis of chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.322 | dTDP-beta-L-vancosamine + devancosaminyl-vancomycin | Amycolatopsis orientalis | natural substrate | dTDP + vancomycin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.310 | Amycolatopsis orientalis | P96559 | - |
- |
| 2.4.1.310 | Amycolatopsis orientalis A82846 | P96559 | - |
- |
| 2.4.1.322 | Amycolatopsis orientalis | Q9AFC7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.310 | - |
Amycolatopsis orientalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.310 | UDP-alpha-D-glucose + teicoplanin aglycone | very low glucosylation activity | Amycolatopsis orientalis | UDP + ? | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + teicoplanin aglycone | very low glucosylation activity | Amycolatopsis orientalis A82846 | UDP + ? | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | - |
Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | - |
Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.310 | UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? | |
| 2.4.1.322 | dTDP-beta-L-vancosamine + devancosaminyl-vancomycin | natural substrate | Amycolatopsis orientalis | dTDP + vancomycin | - |
? | |
| 2.4.1.322 | UDP-4-epi-vancosamine + devancosaminyl-vancomycin | - |
Amycolatopsis orientalis | UDP + epivancomycin | epivancomycin is a hybrid teicoplanin/vancomycin glycopeptide | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.310 | ? | x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE | Amycolatopsis orientalis |
| 2.4.1.322 | ? | x * 44000, SDS-PAGE | Amycolatopsis orientalis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.310 | GtfB | - |
Amycolatopsis orientalis |
| 2.4.1.322 | GtfD | - |
Amycolatopsis orientalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.310 | 37 | - |
assay at | Amycolatopsis orientalis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.310 | 0.283 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis | |
| 2.4.1.310 | 0.283 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.310 | 9 | - |
assay at | Amycolatopsis orientalis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.4.1.310 | physiological function | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.310 | 0.88 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis | |
| 2.4.1.310 | 4.6 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis | |
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