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Literature summary extracted from

  • Zhou, Z.H.; Adams, M.W.
    Site-directed mutations of the 4Fe-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus: role of the cluster-coordinating aspartate in physiological electron transfer reactions (1997), Biochemistry, 36, 10892-10900.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 additional information
-
 additional information pH 8.0, 80°C, kinetic parameters for the native and mutant forms of Pyrococcus furiosus ferredoxin Pyrococcus furiosus
1.2.7.5 additional information
-
 additional information pH 8.0, 80°C, kinetic parameters for the native and mutant forms of Pyrococcus furiosus ferredoxin Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Pyrococcus furiosus
-
-
-
1.2.7.5 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin the ability of the 4Fe-ferredoxin to accept electrons is not absolutely dependent upon Asp14 (of ferredoxin), as this residue can be effectively replaced by Cys. However, the efficiency of electron transfer is compromised if Asp14 is replaced by Ser, or if the 4Fe-cluster is converted to the 3Fe-form, but Asp14 does not appear to offer any kinetic advantage over the expected Cys Pyrococcus furiosus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.5 crotonaldehyde + CoA + 2 oxidized ferredoxin the ability of the 4Fe-ferredoxin to accept electrons is not absolutely dependent upon Asp14 (of ferredoxin), as this residue can be effectively replaced by Cys. However, the efficiency of electron transfer is compromised if Asp14 is replaced by Ser, or if the 4Fe-cluster is converted to the 3Fe-form, but Asp14 does not appear to offer any kinetic advantage over the expected Cys Pyrococcus furiosus crotonate + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 POR
-
 Pyrococcus furiosus
1.2.7.5 POR
-
 Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.7.1 additional information
-
 additional information pH 8.0, 80°C, kinetic parameters for the native and mutant forms of Pyrococcus furiosus ferredoxin Pyrococcus furiosus
1.2.7.5 additional information
-
 additional information pH 8.0, 80°C, kinetic parameters for the native and mutant forms of Pyrococcus furiosus ferredoxin Pyrococcus furiosus