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Literature summary extracted from

  • Rozova, O.N.; Khmelenina, V.N.; Trotsenko, Y.A.
    Characterization of recombinant PPi-dependent 6-phosphofructokinases from Methylosinus trichosporium OB3b and Methylobacterium nodulans ORS 2060 (2012), Biochemistry (Moscow), 77, 288-295.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.90 expressed in Escherichia coli BL21(DE3) cells Methylosinus trichosporium
2.7.1.90 expressed in Escherichia coli BL21(DE3) cells Methylobacterium nodulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 ADP 50% inhibition in the forward reaction and complete inhibition in the reverse reaction at 1 mM Methylosinus trichosporium
2.7.1.90 AMP 90% inhibition in the forward reaction and complete inhibition in the reverse reaction at 1 mM Methylosinus trichosporium
2.7.1.90 additional information not inhibited by ADP or AMP Methylobacterium nodulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.006
-
 diphosphate at pH 7.5 and 30°C Methylobacterium nodulans
2.7.1.90 0.011
-
 diphosphate at pH 7.5 and 30°C Methylosinus trichosporium
2.7.1.90 0.043
-
 D-fructose 1,6-bisphosphate at pH 7.5 and 30°C Methylobacterium nodulans
2.7.1.90 0.07
-
 D-fructose 1,6-bisphosphate at pH 7.5 and 30°C Methylosinus trichosporium
2.7.1.90 0.21
-
 D-sedoheptulose 7-phosphate at pH 7.5 and 30°C Methylobacterium nodulans
2.7.1.90 0.24
-
 D-fructose 6-phosphate at pH 7.5 and 30°C Methylosinus trichosporium
2.7.1.90 0.51
-
 D-sedoheptulose 7-phosphate at pH 7.5 and 30°C Methylosinus trichosporium
2.7.1.90 0.65
-
 D-fructose 6-phosphate at pH 7.5 and 30°C Methylobacterium nodulans
2.7.1.90 1.04
-
 phosphate at pH 7.5 and 30°C Methylobacterium nodulans
2.7.1.90 2.4
-
 phosphate at pH 7.5 and 30°C Methylosinus trichosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ at 1 mM concentration Co2+ can replace Mg2+ (about 50% of Mg2+ activity) Methylobacterium nodulans
2.7.1.90 Mg2+ required, Km values of 0.03 mM (forward reaction) and 0.363 mM (reverse reaction) at pH 7.5 and 50°C Methylobacterium nodulans
2.7.1.90 Mg2+ required, Km values of 0.047 mM (forward reaction) and 0.36 mM (reverse reaction) at pH 7.5 and 30°C Methylosinus trichosporium
2.7.1.90 Mn2+ at 1 mM concentration Mn2+ can replace Mg2+ (81% of Mg2+ activity) Methylosinus trichosporium
2.7.1.90 Mn2+ at 1 mM concentration Mn2+ can replace Mg2+ (about 65% of Mg2+ activity) Methylobacterium nodulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 43000
-
 4 * 43000, SDS-PAGE Methylobacterium nodulans
2.7.1.90 45000
-
 6 * 45000, SDS-PAGE Methylosinus trichosporium
2.7.1.90 180000
-
 gel filtration Methylobacterium nodulans
2.7.1.90 270000
-
 gel filtration Methylosinus trichosporium

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Methylobacterium nodulans B8IF02
-
-
2.7.1.90 Methylobacterium nodulans ORS 2060 B8IF02
-
-
2.7.1.90 Methylosinus trichosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 Ni2+NTA column chromatography, gel filtration Methylosinus trichosporium
2.7.1.90 Ni2+NTA column chromatography, gel filtration Methylobacterium nodulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme is highly specific to diphosphate as the phosphoryl donor Methylosinus trichosporium phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme is highly specific to diphosphate as the phosphoryl donor Methylobacterium nodulans phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme is highly specific to diphosphate as the phosphoryl donor Methylobacterium nodulans ORS 2060 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-ribulose 5-phosphate 1.4% activity compared to D-fructose 6-phosphate Methylosinus trichosporium ?
-
 ?
2.7.1.90 diphosphate + D-sedoheptulose 7-phosphate
-
 Methylosinus trichosporium phosphate + D-sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-sedoheptulose 7-phosphate
-
 Methylobacterium nodulans phosphate + D-sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-sedoheptulose 7-phosphate
-
 Methylobacterium nodulans ORS 2060 phosphate + D-sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 additional information D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme Methylosinus trichosporium ?
-
 ?
2.7.1.90 additional information D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme. No activity is observed with ribulose 5-phosphate Methylobacterium nodulans ?
-
 ?
2.7.1.90 additional information D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme. No activity is observed with ribulose 5-phosphate Methylobacterium nodulans ORS 2060 ?
-
 ?
2.7.1.90 phosphate + D-fructose 1,6-bisphosphate
-
 Methylosinus trichosporium diphosphate + D-fructose 6-phosphate
-
 r
2.7.1.90 phosphate + D-fructose 1,6-bisphosphate
-
 Methylobacterium nodulans diphosphate + D-fructose 6-phosphate
-
 r
2.7.1.90 phosphate + D-fructose 1,6-bisphosphate
-
 Methylobacterium nodulans ORS 2060 diphosphate + D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 homohexamer 6 * 45000, SDS-PAGE Methylosinus trichosporium
2.7.1.90 homotetramer 4 * 43000, SDS-PAGE Methylobacterium nodulans

Synonyms

EC Number Synonyms Comment Organism
2.7.1.90 PPi-dependent 6-phosphofructokinase
-
 Methylosinus trichosporium
2.7.1.90 PPi-dependent 6-phosphofructokinase
-
 Methylobacterium nodulans
2.7.1.90 PPi-PFK
-
 Methylosinus trichosporium
2.7.1.90 PPi-PFK
-
 Methylobacterium nodulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 30
-
-
 Methylobacterium nodulans
2.7.1.90 50
-
-
 Methylosinus trichosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.5
-
-
 Methylosinus trichosporium
2.7.1.90 7.5
-
-
 Methylobacterium nodulans