EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.85 | gene SlSPS, recombinant expression in Escherichia coli. In planta, constitutive overexpression of SlSPS elevates the plastoquinone content of immature tobacco leaves | Solanum lycopersicum |
2.5.1.86 | gene SlDPS, recombinant expression in Escherichia coli | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.85 | additional information | virus-induced gene silencing. The gene SlSPS is not able to complement silencing of gene SlDPS, encoding a decaprenyl diphosphate synthase, EC 2.5.1.86 | Solanum lycopersicum |
2.5.1.86 | additional information | virus-induced gene silencing. The gene SlSPS is not able to complement silencing of gene SlSPS, encoding a solanesyl diphosphate synthase, EC 2.5.1.85 | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.B14 | 0.00046 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Solanum lycopersicum | |
2.5.1.B14 | 0.00151 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Solanum lycopersicum | |
2.5.1.91 | 0.00418 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.5, 30°C | Solanum lycopersicum | |
2.5.1.91 | 0.00863 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.B14 | plastid | product solanesol is associated with thylakoid membranes | Solanum lycopersicum | 9536 | - |
2.5.1.85 | chloroplast | - |
Solanum lycopersicum | 9507 | - |
2.5.1.85 | additional information | solanesyl diphosphate synthase is targeted to the plastid and both solanesol and plastoquinone are associated with thylakoid membranes | Solanum lycopersicum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.91 | Mg2+ | and Mn2+, effective in stimulating the activity | Solanum lycopersicum | |
2.5.1.91 | Mn2+ | and Mn2+, effective in stimulating the activity | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.91 | 45443 | - |
x * 45443, calculated | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | additional information | Solanum lycopersicum | the enzyme can utilize geranyl, farnesyl or geranylgeranyl diphosphates in the synthesis of C45 and C50 prenyl diphosphates | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.B14 | Solanum lycopersicum | Q09IV6 | - |
- |
2.5.1.85 | Solanum lycopersicum | - |
gene SlSPS | - |
2.5.1.86 | Solanum lycopersicum | - |
gene SlDPS | - |
2.5.1.91 | Solanum lycopersicum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.B14 | (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | vmax/Km for (2E,6E)-farnesyl diphosphate is 2.8fold higher than vmax/Km for geranylgeranyl diphosphate | Solanum lycopersicum | 6 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.B14 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | vmax/Km for (2E,6E)-farnesyl diphosphate is 2.8fold higher than vmax/Km for geranylgeranyl diphosphate | Solanum lycopersicum | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | enzyme SlSPS extends the prenyl chain length of the endogenous ubiquinone to nine isoprene units | Solanum lycopersicum | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | enzyme SlDPS extends the prenyl chain length of the endogenous ubiquinone to ten isoprene units | Solanum lycopersicum | 7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | additional information | the enzyme can utilize geranyl, farnesyl or geranylgeranyl diphosphates in the synthesis of C45 and C50 prenyl diphosphates | Solanum lycopersicum | ? | - |
? | |
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Solanum lycopersicum | 7 diphosphate + decaprenyl diphosphate | plus solanesyl diphosphate | ? | |
2.5.1.91 | (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Solanum lycopersicum | diphosphate + ? | main products are decaprenyl diphosphate and solanesyl diphosphate | ? | |
2.5.1.91 | geranyl diphosphate + isopentenyl diphosphate | - |
Solanum lycopersicum | diphosphate + ? | main products are decaprenyl diphosphate and solanesyl diphosphate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.91 | ? | x * 45443, calculated | Solanum lycopersicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.B14 | SPPS | - |
Solanum lycopersicum |
2.5.1.B14 | SPS | - |
Solanum lycopersicum |
2.5.1.85 | long-chain prenyl diphosphate synthase | - |
Solanum lycopersicum |
2.5.1.85 | SlSPS | - |
Solanum lycopersicum |
2.5.1.85 | solanesyl diphosphate synthase | - |
Solanum lycopersicum |
2.5.1.86 | decaprenyl diphosphate synthase | - |
Solanum lycopersicum |
2.5.1.86 | long-chain prenyl diphosphate synthase | - |
Solanum lycopersicum |
2.5.1.86 | SlDPS | - |
Solanum lycopersicum |
2.5.1.91 | Dps | - |
Solanum lycopersicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.B14 | physiological function | when expressed in Escherichia coli, enzyme extends the prenyl chain length of the endogenous ubiquinone to nine isoprene units respectively. In planta, constitutive overexpression elevates the plastoquinone content of immature tobacco leaves. Virus-induced gene silencing shows that the enzyme is necessary for normal chloroplast structure and function. Plants silenced for SPS are photobleached and accumulate phytoene | Solanum lycopersicum |
2.5.1.85 | malfunction | plants silenced for SlSPS are photobleached and accumulate phytoene | Solanum lycopersicum |
2.5.1.85 | metabolism | requirement for two long-chain prenyl diphosphate synthases in the tomato, i.e. SlSPS and SlDPS, a decaprenyl diphosphate synthase, EC 2.5.1.86 | Solanum lycopersicum |
2.5.1.85 | physiological function | enzyme SlSPS is necessary for normal chloroplast structure and function | Solanum lycopersicum |
2.5.1.86 | malfunction | plants silenced for SlSPS are not affected in leaf appearance, but impacted on primary metabolism | Solanum lycopersicum |
2.5.1.86 | metabolism | requirement for two long-chain prenyl diphosphate synthases in the tomato, i.e. SlSPS, a solanesyl diphosphate synthase, EC 2.5.1.85, and SlDPS, a decaprenyl diphosphate synthase | Solanum lycopersicum |